Yagi K, Ikeda S, Schweiss J F, Homan S M
Department of Anesthesiology, St. Louis University School of Medicine, Missouri 63110-0250.
Anesthesiology. 1990 Nov;73(5):1028-31. doi: 10.1097/00000542-199011000-00032.
The use of a cyanide ion-specific electrode in combination with the Conway microdiffusion method was modified for the measurement of cyanide concentration in human red blood cells and plasma. With our modified method, the optimal pH of cyanide isolation from red blood cells and plasma was investigated. Cyanide recovery from red blood cells increased with decreasing pH. The maximal recovery of 96.9 +/- 2.6% was obtained at a pH of less than 1. Cyanide recovery from plasma, however, peaked at a pH between 7 and 8, and further changes in pH reduced the recovery rate. The maximal recovery rate from plasma was 74.1 +/- 1.5%. In previous studies, cyanide isolations from both plasma and red blood cells were carried out at a pH of less than 1. This study shows that cyanide isolation from plasma should be performed at a pH between 7 and 8.
将氰离子特异性电极与康威微量扩散法结合使用，对其进行了改进，用于测量人体红细胞和血浆中的氰化物浓度。采用我们改进的方法，研究了从红细胞和血浆中分离氰化物的最佳pH值。从红细胞中回收氰化物的量随pH值降低而增加。在pH值小于1时，最大回收率为96.9±2.6%。然而，从血浆中回收氰化物的量在pH值为7至8时达到峰值，pH值的进一步变化会降低回收率。血浆的最大回收率为74.1±1.5%。在以往的研究中，从血浆和红细胞中分离氰化物均在pH值小于1的条件下进行。本研究表明，从血浆中分离氰化物应在pH值为7至8的条件下进行。
