el-Awar F Y, Ochs D L, Pyle R H, Misra H P
Department of Biomedical Sciences, Virginia-Maryland Regional College of Veterinary Medicine, Virginia Polytechnic Institute and State University, Blacksburg 24061.
Am J Vet Res. 1990 Oct;51(10):1561-4.
The response of blood neutrophils to the chemotactic peptide formyl-methyl-leucyl-phenylalanine varies among species. Our results indicate that this peptide does not activate the respiratory burst of porcine neutrophils. Specifically, concentrations less than or equal to 10(-6) M did not cause production of either superoxide or hydrogen peroxide. Studies designed to delineate the biochemical deficit responsible for these results indicated that these cells do not express specific chemotactic peptide receptors on the external surface of the plasma membrane. Although these data do not rule out the possibility that internal stores of chemotactic peptide receptor exist, attempts to induce expression of the receptor by priming the cells with either lipopolysaccharide or calcium ionophore were unsuccessful.
血液中性粒细胞对趋化肽甲酰 - 甲基 - 亮氨酰 - 苯丙氨酸的反应因物种而异。我们的结果表明，该肽不会激活猪中性粒细胞的呼吸爆发。具体而言，浓度小于或等于10^(-6) M的该肽不会导致超氧化物或过氧化氢的产生。旨在确定导致这些结果的生化缺陷的研究表明，这些细胞在质膜外表面不表达特定的趋化肽受体。尽管这些数据不排除存在趋化肽受体内部储存库的可能性，但用脂多糖或钙离子载体预处理细胞以诱导受体表达的尝试均未成功。
