Sun Jiao, Xie Guangping
Shanghai Biomaterials Research and Testing Center Ninth People's Hospital, Shanghai JiaoTong University School of Medicine, Shanghai 200023, China.
J Nanosci Nanotechnol. 2011 Dec;11(12):10996-1000. doi: 10.1166/jnn.2011.3956.
Hydroxyapatite nanoparticles (HANPs) have been developed for biomedical use due to its extraordinary properties. However, the side effects of HANPs on human body have also been concerned, especially in vivo. Now it is still unknown how about the distribution and biobehavior of HANPs in vivo is, though it's very important for application in biosystem. This study was to establish a new method of 125I radiolabeling on HANPs at 80 nm, investigate the long-term tissue distribution of HANPs quantitatively after intravenously administrated HANPs labeled with 125I and the subcellular distribution in liver and spleen by TEM. The results indicated the labeled HANPs had high stability in vitro, and could accumulate mainly in the liver and spleen and decrease in time dependent manner, but still retain in body for more than 28 days. This stagnation most probably attribute to the endocytosis by macrophages in these tissues. The results implied the radioactive iodine labeling was an effective and sensitive method for tracing and analyzing the distribution of NPs in vivo. Liver and spleen should be the main target organ reached when HANPs were injected into circulation system. Because HANPs could stay in vivo for over one month, the biosafety shouldn't be neglected.
羟基磷灰石纳米颗粒（HANPs）因其卓越的性能已被开发用于生物医学领域。然而，HANPs对人体的副作用也受到关注，尤其是在体内。目前，尽管HANPs在生物系统中的应用非常重要，但其在体内的分布和生物行为仍不清楚。本研究旨在建立一种对80 nm的HANPs进行¹²⁵I放射性标记的新方法，定量研究¹²⁵I标记的HANPs静脉注射后在体内的长期组织分布以及通过透射电子显微镜（TEM）观察其在肝脏和脾脏中的亚细胞分布。结果表明，标记后的HANPs在体外具有高稳定性，主要在肝脏和脾脏中蓄积，并呈时间依赖性减少，但在体内仍可保留超过28天。这种滞留很可能归因于这些组织中巨噬细胞的内吞作用。结果表明，放射性碘标记是一种有效且灵敏的方法，可用于追踪和分析纳米颗粒在体内的分布。当HANPs注入循环系统时，肝脏和脾脏应是主要到达的靶器官。由于HANPs可在体内停留一个多月，其生物安全性不容忽视。
