Department of Chemistry, Faculty of Science, Yazd University, Yazd, 89195-741, Islamic Republic of Iran.
Department of Chemistry, Faculty of Science, Yazd University, Yazd, 89195-741, Islamic Republic of Iran.
Biosens Bioelectron. 2012 May 15;35(1):75-81. doi: 10.1016/j.bios.2012.02.014. Epub 2012 Feb 17.
In the present paper, the use of a carbon paste electrode modified by meso-tetrakis(3-methylphenyl) cobalt porphyrin (CP) and TiO(2) nanoparticles for the determination of levodopa (LD) and carbidopa (CD) was described. Initially, cyclic voltammetry was used to investigate the redox properties of this modified electrode at various scan rates. Next, the mediated oxidation of LD at the modified electrode was described. At the optimum pH of 7.0, the oxidation of LD occurs at a potential about 150 mV less positive than that of an unmodified carbon paste electrode. Based on differential pulse voltammetry (DPV), the oxidation of LD exhibited a dynamic range between 0.1 and 100.0 μM and a detection limit (3σ) of 69 ± 2 nM. DPV was used for simultaneous determination of LD and CD at the modified electrode, and quantitation of LD and CD in some real samples (such as tablets of Parkin-C Fort and Madopar, water, urine, and human blood serum) by the standard addition method.
本文描述了一种使用介孔四(3-甲基苯基)钴卟啉(CP)和 TiO2纳米粒子修饰的碳糊电极来测定左旋多巴(LD)和卡比多巴(CD)的方法。首先，在不同的扫描速率下，通过循环伏安法研究了该修饰电极的氧化还原性质。接着，描述了 LD 在修饰电极上的介导氧化。在最佳 pH 值 7.0 下，LD 的氧化发生在比未修饰的碳糊电极正约 150 mV 的电位处。基于差分脉冲伏安法(DPV)，LD 的氧化在 0.1 到 100.0 μM 之间呈现动态范围，检测限(3σ)为 69 ± 2 nM。DPV 可用于在修饰电极上同时测定 LD 和 CD，并通过标准加入法对一些实际样品(如 Parkin-C Fort 和 Madopar 片剂、水、尿液和人血清)中的 LD 和 CD 进行定量。
