Beckman Institute, Division of Biology, California Institute of Technology, Pasadena, California 91125, USA.
Invest Ophthalmol Vis Sci. 2012 Apr 30;53(4):2337-48. doi: 10.1167/iovs.11-7823.
To investigate the retinal and choroidal vascular pattern, structure, and thickness using high-speed, high axial resolution, swept-source optical coherence tomography (SS-OCT) at 1060 nm, demonstrating enhanced penetration through all choroidal layers.
An ophthalmic SS-OCT system was developed operating at 57,000 A-lines/s with 5.9 μm axial resolution and was used to collect 3D images with scanning angles up to ∼70° × 35°. The similar features were observed in the choroidal layers by imaging three healthy volunteers. En face images, extracted at different depths, capture features of the retinal and choroidal vasculature networks and substructure. Retinal and choroidal thicknesses were measured over scanning angles of ∼14° × 14°, yielding retinal and choroidal thickness maps.
The retinal layers, choriocapillaris (CC), Sattler's layer (SL), Haller's layer (HL), and the lamina suprachoroid layer (LSL) were delineated in 2D sagittal tomograms. The sagittal tomograms and en face reflectance images over a 2-cm(2) field of view captured the paraoptic, lateral and medial distal short posterior ciliary artery (SPCA) branches as well as the two lateral and medial long posterior ciliary arteries (LPCAs). En face images in the HL revealed the superotemporal, inferotemporal, superonasal, and inferonasal major choroidal vessels.
High-speed, high-resolution SS-OCT centered at 1060 nm enables retinal and choroidal vasculature networks visualization, including retina vessels, posterior ciliary artery (PCA) branches, and venous vascular patterns. This technology offers diagnostic opportunities by monitoring change in these networks, substructure, and retinal and choroidal thicknesses during disease initiation and progression.
利用高速、高轴向分辨率的扫频源光学相干断层扫描（SS-OCT）在 1060nm 处探测视网膜和脉络膜的血管形态、结构和厚度，显示出对所有脉络膜层的增强穿透能力。
开发了一种眼科 SS-OCT 系统，以 57000A-lines/s 的速度运行，轴向分辨率为 5.9μm，并使用高达约 70°×35°的扫描角度来采集 3D 图像。对 3 名健康志愿者进行成像，观察到脉络膜层具有相似的特征。在不同深度提取的共焦图像，捕捉到视网膜和脉络膜血管网络及其亚结构的特征。在约 14°×14°的扫描角度上测量视网膜和脉络膜的厚度，生成视网膜和脉络膜的厚度图。
在二维矢状断层图像中，可描绘出视网膜各层、脉络膜毛细血管（CC）、Sattler 层（SL）、Haller 层（HL）和脉络膜上腔层（LSL）。2cm²视场的矢状断层图像和共焦反射图像捕捉到视旁、外侧和内侧远后睫状短动脉（SPCA）分支，以及两条外侧和内侧长后睫状动脉（LPCAs）。HL 的共焦图像显示了上颞、下颞、上鼻和下鼻主要脉络膜血管。
中心波长为 1060nm 的高速、高分辨率 SS-OCT 可实现视网膜和脉络膜血管网络的可视化，包括视网膜血管、睫状后动脉（PCA）分支和静脉血管模式。这项技术提供了监测这些网络、亚结构以及视网膜和脉络膜厚度在疾病发生和进展过程中变化的诊断机会。
