Department of Pathology, New York University School of Medicine, New York, NY, USA.
EMBO J. 2012 Apr 4;31(7):1627-9. doi: 10.1038/emboj.2012.64. Epub 2012 Mar 13.
EMBO J 31 7, 1666–1678 (2012); February 28 2012 By sequencing tens of millions of TCRα transcripts from naive mouse CD8 T cells, Genolet et al (2012) show that the TCRα repertoire is at least as diverse as the TCRβ repertoire. This overturns a long held view in the field that recombination of the locus occurs in a co-ordinate sequential bidirectional manner that relies on proximity of Vα and Jα gene segments. The observation that rearrangement of all possible Vα-Jα combinations can occur is consistent with an alternative model in which intralocus loop formation/locus contraction enables an opportunity for all Vα gene segments to recombine with Jα gene segments.
EMBO J 31 7, 1666–1678 (2012); February 28 2012 通过对幼稚型 CD8 T 细胞的 TCRα 转录本进行数十亿次测序，Genolet 等人（2012 年）表明 TCRα 库的多样性至少与 TCRβ 库相当。这推翻了该领域长期以来的一个观点，即 基因座的重组以协调的顺序双向方式发生，依赖于 Vα 和 Jα 基因片段的接近性。所有可能的 Vα-Jα 组合都能发生重排的观察结果与替代模型一致，即基因内环形成/基因座收缩使所有 Vα 基因片段有机会与 Jα 基因片段发生重组。
