Malki Idir, Cardoso de Amorim Gisele, Simenel Catherine, Prochnicka-Chalufour Ada, Delepierre Muriel, Izadi-Pruneyre Nadia
Institut Pasteur, Unité de RMN des Biomolécules, Département de Biologie Structurale et Chimie, 28, rue du Dr Roux, 75724, Paris Cedex 15, France.
Biomol NMR Assign. 2013 Apr;7(1):43-6. doi: 10.1007/s12104-012-9377-y. Epub 2012 Mar 14.
TonB-dependent transporters (TBDTs) are bacterial outer membrane proteins that internalize nutrients such as vitamin B12, metal complexes, heme, some carbohydrates, etc. In addition to their transport activity, several TBDTs are also involved in a signalling cascade from the cell surface into the cytoplasm, via their periplasmic signalling domain. Here we report the backbone and side chain resonance assignments of the signalling domain of HasR, a TonB-dependent outer membrane heme transporter from Serratia marcescens as a first step towards its structural study.
托蛋白B依赖型转运蛋白（TBDTs）是细菌外膜蛋白，可将维生素B12、金属络合物、血红素、某些碳水化合物等营养物质转运至细胞内。除了转运活性外，一些TBDTs还通过其周质信号结构域参与从细胞表面到细胞质的信号级联反应。在此，我们报道了粘质沙雷氏菌的托蛋白B依赖型外膜血红素转运蛋白HasR信号结构域的主链和侧链共振归属，这是对其进行结构研究的第一步。
