Dept. of Marine Food Science and Technology, Gangneung-Wonju National Univ., Gangneung 210-702, Republic of Korea.
J Food Sci. 2011 Nov-Dec;76(9):H208-14. doi: 10.1111/j.1750-3841.2011.02391.x. Epub 2011 Oct 4.
One therapeutic approach for preventing diabetes mellitus and obesity is to retard the absorption of glucose via inhibition of α-glucosidase. Two unsaturated fatty acids with strong α-glucosidase inhibitory activity, 7(Z)-octadecenoic acid (1) and 7(Z),10(Z)-octadecadienoic acid (2), were purified from the body wall of Stichopus japonicus. IC(50) values of compounds 1 and 2 were 0.51 and 0.67 μg/mL against Saccharomyces cerevisiae α-glucosidase and 0.49 and 0.60 μg/mL against Bacillus stearothermophilus α-glucosidase, respectively. These compounds mildly inhibited rat-intestinal sucrase and maltase. In addition, both compounds showed a mixed type of inhibition against S. cerevisiae α-glucosidase and were very stable under thermal and acidic conditions up to 60 min. The K(I) and K(IS) values of compounds 1 and 2 were 0.44 and 0.22 μg/mL, and 0.39 and 0.13 μg/mL, respectively.
One therapeutic approach for preventing diabetes mellitus is to retard the absorption of glucose via inhibition of α-glucosidase. In this study, 2 fatty acids with strong α-glucosidase-inhibitory activity, 7(Z)-octadecenoic acid and 7(Z),10(Z)-octadecadienoic acid, were purified and identified from sea cucumber. Therefore, sea cucumber fatty acids can potentially be developed as a novel natural nutraceutical for the management of type-2 diabetes.
预防糖尿病和肥胖的一种治疗方法是通过抑制α-葡萄糖苷酶来延缓葡萄糖的吸收。两种具有强烈α-葡萄糖苷酶抑制活性的不饱和脂肪酸，7(Z)-十八碳烯酸（1）和 7(Z)，10(Z)-十八碳二烯酸（2），从刺参体壁中纯化得到。化合物 1 和 2 对酿酒酵母α-葡萄糖苷酶的 IC50 值分别为 0.51 和 0.67 μg/mL，对嗜热脂肪芽孢杆菌α-葡萄糖苷酶的 IC50 值分别为 0.49 和 0.60 μg/mL。这些化合物对大鼠肠蔗糖酶和麦芽糖酶有轻微抑制作用。此外，两种化合物对酿酒酵母α-葡萄糖苷酶均表现出混合抑制作用，在 60 分钟内的热和酸性条件下非常稳定。化合物 1 和 2 的 K(I)和 K(IS)值分别为 0.44 和 0.22 μg/mL，0.39 和 0.13 μg/mL。
预防糖尿病的一种治疗方法是通过抑制α-葡萄糖苷酶来延缓葡萄糖的吸收。在这项研究中，从海参中分离和鉴定出两种具有强α-葡萄糖苷酶抑制活性的脂肪酸，7(Z)-十八碳烯酸和 7(Z)，10(Z)-十八碳二烯酸。因此，海参脂肪酸有可能被开发为一种新型天然营养保健品，用于 2 型糖尿病的管理。
