Pedaci Francesco, Huang Zhuangxiong, van Oene Maarten, Dekker Nynke H
Department of Bionanoscience, Kavli Institute of Nanoscience, Faculty of Applied Sciences, Delft University of Technology, Lorentzweg 1, 2628 CJ Delft, The Netherlands.
Opt Express. 2012 Feb 13;20(4):3787-802. doi: 10.1364/OE.20.003787.
The optical torque wrench is a laser trapping technique that expands the capability of standard optical tweezers to torque manipulation and measurement, using the laser linear polarization to orient tailored microscopic birefringent particles. The ability to measure torque of the order of kBT (∼4 pN nm) is especially important in the study of biophysical systems at the molecular and cellular level. Quantitative torque measurements rely on an accurate calibration of the instrument. Here we describe and implement a set of calibration approaches for the optical torque wrench, including methods that have direct analogs in linear optical tweezers as well as introducing others that are specifically developed for the angular variables. We compare the different methods, analyze their differences, and make recommendations regarding their implementations.
光学扭矩扳手是一种激光捕获技术，它利用激光线性偏振使定制的微观双折射粒子定向，从而将标准光镊的功能扩展到扭矩操纵和测量。在分子和细胞水平的生物物理系统研究中，测量约kBT（~4 pN nm）量级扭矩的能力尤为重要。定量扭矩测量依赖于仪器的精确校准。在此，我们描述并实施了一套光学扭矩扳手的校准方法，包括在线性光镊中有直接类似方法的，以及专门为角度变量开发的其他方法。我们比较了不同方法，分析了它们的差异，并就其实施提出了建议。
