Rad Isa Abdi, Bagheri Morteza, Rahimi-Rad Mohammad Hosein
Cellular and Molecular Research Center, Urmia University of Medical Sciences, Iran.
Pneumologia. 2011 Oct-Dec;60(4):208-12.
Angiotensin-converting enzyme (ACE) has an important role in inactivation of bradykinin and tachykinins which known as powerful bronchoconstrictors. It has been demonstrated that an insertion (1)/deletion (D) genetic variations within the ACE gene greatly influence the plasma level of ACE.
The aim of the present study was to determine the frequencies of ACE D and I alleles and ACE DD, DI and II genotypes in asthmatic patients and controls with Iranian Azeri-Turkish origin and to compare the frequency of the ACE genotypes between asthmatic patients and controls.
We genotyped 212 healthy controls including 73 males and 138 females, as well as 62 patients with asthma, including 28 males and 34 females by PCR.
Of the 212 healthy controls: 1) the prevalence of DD, DI, and II genotypes were 83(39.151), 92(43.396) and 37(17.453), respectively. 2) the frequency (%) was 257(60.9)for D allele and 165(39.1 )for II allele. 3) D and II allele frequencies were 0.61 and 0.39 respectively. Of the 62 patients with asthma: 1) the prevalence of DD, II, and III genotypes were 17(27.42), 31(50) and 14(22.58), respectively. 2) the frequency (%) was 65(52.42)for D allele and 59(47.58 ) for II allele. 3) D and II allele frequencies were 0.52 and 0.48 respectively. Statistical analysis showed that studied groups (female + male patients group and female + male controls group) were in Hardy-Weinberg equilibrium. Our findings imply that II/D ratio was 0.61/0.39 in all controls and 0.6/0.4 in male or female controls. Significant differences were not found in the ACE genotype or allele frequencies between studied groups regarding all cases versus all controls, female cases versus female controls, male cases versus male controls.
We have concluded that deletion allele of the ACE gene is not a risk factor for asthma predisposition.
血管紧张素转换酶（ACE）在缓激肽和速激肽（已知为强大的支气管收缩剂）的失活中起重要作用。已证明ACE基因内的插入（I）/缺失（D）基因变异极大地影响ACE的血浆水平。
本研究的目的是确定伊朗阿塞拜疆 - 土耳其裔哮喘患者和对照中ACE D和I等位基因以及ACE DD、DI和II基因型的频率，并比较哮喘患者和对照中ACE基因型的频率。
我们通过PCR对212名健康对照（包括73名男性和138名女性）以及62名哮喘患者（包括28名男性和34名女性）进行基因分型。
在212名健康对照中：1）DD、DI和II基因型的患病率分别为83（39.151）、92（43.396）和37（17.453）。2）D等位基因的频率（%）为257（60.9），I等位基因的频率为165（39.1）。3）D和I等位基因频率分别为0.61和0.39。在62名哮喘患者中：1）DD、II和III基因型的患病率分别为17（27.42）、31（50）和14（22.58）。2）D等位基因的频率（%）为65（52.42），I等位基因的频率为59（47.58）。3）D和I等位基因频率分别为0.52和0.48。统计分析表明，研究组（女性+男性患者组和女性+男性对照组）处于哈迪 - 温伯格平衡。我们的研究结果表明，所有对照组中I/D比值为0.61/0.39，男性或女性对照组中为0.6/0.4。在所有病例与所有对照、女性病例与女性对照、男性病例与男性对照的研究组之间，未发现ACE基因型或等位基因频率存在显著差异。
我们得出结论，ACE基因的缺失等位基因不是哮喘易感性的危险因素。
