Department of Surgery, Korea University College of Medicine, Seoul, Korea.
J Surg Oncol. 2012 Sep 15;106(4):456-61. doi: 10.1002/jso.23097. Epub 2012 Mar 15.
The male predominance of gastric cancer suggests that female sex hormones may have a protective effect against gastric cancer. We evaluated the expression of estrogen receptors in gastric cancer tissue and cells and the clinical significance of ER-β expression in gastric cancer.
ER-α, ER-β proteins extracted from normal stomach, gastric cancer tissues, and cultured gastric cancer cells (KATO-III, mkn28, mkn45, and mkn74) were assessed by Western blot analysis. The clinical significance of ER-β was explored using tissue microarray methods and immunohistochemical staining of specimens from 148 gastric cancers.
Both ER-α and β protein expression were noted in normal and gastric cancer tissues. However, in cultured gastric cells, only ER-β was noted in mkn28 and mkn74. Of 148 gastric cancers, 67 (45.3%) were ER-β positive. The ER-β positive group was associated with lower tumor stage, Lauren's intestinal type, negative perineural invasion, and free of recurrence. The ER-β positive group had a better 3-year survival compared with the negative group in survival analysis.
Our results suggest that the presence of ER-β in gastric cancer could have a protective effect against invasiveness of gastric cancer. Further studies are needed to clarify the role of ER-β in gastric cancers.
胃癌的男性高发倾向提示女性性激素可能对胃癌具有保护作用。我们评估了胃癌组织和细胞中雌激素受体的表达情况，以及 ER-β 在胃癌中的表达的临床意义。
采用 Western blot 分析方法检测正常胃组织、胃癌组织和培养的胃癌细胞（KATO-III、mkn28、mkn45 和 mkn74）中 ER-α、ER-β 蛋白的表达。采用组织微阵列方法和免疫组化染色对 148 例胃癌标本进行 ER-β 表达的临床意义研究。
正常和胃癌组织中均有 ER-α 和 β 蛋白表达，但在培养的胃癌细胞中，仅在 mkn28 和 mkn74 中发现 ER-β。在 148 例胃癌中，67 例（45.3%）为 ER-β 阳性。与阴性组相比，ER-β 阳性组肿瘤分期较低、Lauren 肠型、无神经周围浸润、无复发。生存分析显示，ER-β 阳性组的 3 年生存率优于阴性组。
我们的研究结果表明，胃癌中 ER-β 的存在可能对胃癌的侵袭性具有保护作用。需要进一步研究来阐明 ER-β 在胃癌中的作用。
