评论“人类转录组中广泛存在的 RNA 和 DNA 序列差异”。
Comment on "Widespread RNA and DNA sequence differences in the human transcriptome".
机构信息
Department of Human Genetics, McGill University, Montreal, Quebec, Canada.
出版信息
Science. 2012 Mar 16;335(6074):1302; author reply 1302. doi: 10.1126/science.1209658.
Li et al. (Research Articles, 1 July 2011, p. 53; published online 19 May 2011) reported large numbers of differences between DNA and messenger RNA in human cells, indicating unprecedented levels of RNA editing, and including sequence changes not produced by any of the known RNA editing mechanisms. However, common sources of systematic errors in high-throughput sequencing technology, which were not properly accounted for in this study, explain most of the claimed differences.
李等人（研究文章，2011 年 7 月 1 日，第 53 页；网上发表于 2011 年 5 月 19 日）报道了人类细胞中 DNA 和信使 RNA 之间大量的差异，表明 RNA 编辑达到了前所未有的水平，并且包括了任何已知的 RNA 编辑机制都无法产生的序列变化。然而，高通量测序技术中常见的系统性误差源在这项研究中没有得到妥善考虑，这些误差源解释了大部分声称的差异。
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