Yang Yuxi, Sakurai Masayuki
Research Institute for Biomedical Sciences, Tokyo University of Science, Chiba, Japan.
Wiley Interdiscip Rev RNA. 2025 Mar-Apr;16(2):e70014. doi: 10.1002/wrna.70014.
Adenosine-to-inosine (A-to-I) RNA editing is a key post-transcriptional modification that influences gene expression and various cellular processes. Advances in sequencing technologies have greatly contributed to the identification of A-to-I editing sites, providing insights into their distribution across coding and non-coding regions. These developments have facilitated the discovery of functionally relevant editing events and have advanced the understanding of their biological roles. This review presents the evolution of methodologies for RNA editing detection and examines recent advances, including chemically-assisted, enzyme-assisted, and quantitative approaches. By evaluating these techniques, we aim to help researchers select the most effective tools for investigating RNA editing and its broader implications in health and disease.
腺苷到次黄苷（A-to-I）RNA编辑是一种关键的转录后修饰，它影响基因表达和各种细胞过程。测序技术的进步极大地促进了A-to-I编辑位点的识别，为了解它们在编码和非编码区域的分布提供了线索。这些进展推动了功能相关编辑事件的发现，并加深了对其生物学作用的理解。本综述介绍了RNA编辑检测方法的演变，并探讨了近期的进展，包括化学辅助、酶辅助和定量方法。通过评估这些技术，我们旨在帮助研究人员选择最有效的工具来研究RNA编辑及其在健康和疾病中的更广泛影响。
