Department of Human Genetics, University of Chicago, Chicago, IL 60637, USA.
Science. 2012 Mar 16;335(6074):1302; author reply 1302. doi: 10.1126/science.1210484.
Li et al. (Research Articles, 1 July 2011, p. 53; published online 19 May 2011) reported more than 10,000 mismatches between messenger RNA and DNA sequences from the same individuals, which they attributed to previously unrecognized mechanisms of gene regulation. We found that at least 88% of these sequence mismatches can likely be explained by technical artifacts such as errors in mapping sequencing reads to a reference genome, sequencing errors, and genetic variation.
李等人(研究文章,2011 年 7 月 1 日,第 53 页;在线发表于 2011 年 5 月 19 日)报道了超过 10000 个来自同一个体的信使 RNA 和 DNA 序列之间的不匹配,他们将这些不匹配归因于以前未被识别的基因调控机制。我们发现,至少 88%的这些序列不匹配可能可以用技术伪影来解释,例如测序读取映射到参考基因组、测序错误和遗传变异中的错误。