Institute of Polymer Research, Waterloo Institute for Nanotechnology, Department of Chemistry, University of Waterloo , Waterloo ON N2L 3G1, Canada.
Langmuir. 2012 Apr 24;28(16):6527-38. doi: 10.1021/la2047646. Epub 2012 Mar 16.
This report highlights some of the recent developments that have been made in the quantitative analysis of fluorescence decays acquired with pyrene-labeled macromolecules. With these new analytical tools, macromolecules of different composition and architecture can now be labeled in a variety of ways with the pyrene chromophore, and the kinetics of pyrene excimer formation can be described to retrieve quantitative information about the internal dynamics of the macromolecules studied. In particular, this review presents the procedure that was followed to develop these new analytical tools and how the process of pyrene excimer formation with vinyl polymers, poly(L-glutamic acid), dendrimers, associative polymers, surfactants, and lipids labeled with pyrene has been successfully characterized thanks to these analysis programs.
本报告重点介绍了在使用芘标记的大分子进行荧光衰减定量分析方面的一些最新进展。有了这些新的分析工具，现在可以用芘发色团以各种方式标记不同组成和结构的大分子，并且可以描述芘激基复合物形成的动力学，以获取有关所研究大分子内部动力学的定量信息。具体来说，本综述介绍了开发这些新分析工具的过程，以及如何成功地利用这些分析程序对带有芘的乙烯基聚合物、聚（L-谷氨酸）、树枝状大分子、缔合聚合物、表面活性剂和脂质进行芘激基复合物形成过程进行表征。
