UPMC Univ Paris06, CNRS, Chimie de la Matière Condensée de Paris, Collège de France, F-75005 Paris, France.
Biomaterials. 2012 Jun;33(17):4431-42. doi: 10.1016/j.biomaterials.2012.03.004. Epub 2012 Mar 17.
The long-term fate of fluorescent non-porous FITC-SiO(2) nanoparticles of various sizes (10-200 nm) and charge is studied in the presence of human dermal fibroblasts. Particle aggregates are formed in the culture medium and uptaken, at least partially, by macropinocytosis. The smallest particles have a strong impact on cell viability and genotoxic effects can be observed for negatively-charged colloids 10 nm in size. Largest particles do not impact on cellular activity and can be monitored in cellulo via fluorescence and transmission electron microscopy studies over two weeks. These observations reveal a significant decrease in the size of silica particles located in endocytic vesicles. The dissolution process is confirmed by monitoring the cell culture medium that contains both colloidal and soluble silica species. Such dissolution can be explained on the sole basis of silica solubility and has great implication for the use of non-porous silica particles as intra-cellular drug release systems.
研究了不同大小（10-200nm）和电荷的荧光非多孔 FITC-SiO2 纳米颗粒在人真皮成纤维细胞中的长期命运。在培养基中形成颗粒聚集体，并通过巨胞饮作用至少部分摄取。最小的颗粒对细胞活力有强烈影响，并且对于大小为 10nm 的带负电荷的胶体可以观察到遗传毒性作用。最大的颗粒不会影响细胞活性，可以通过荧光和透射电子显微镜研究在细胞内监测两周。这些观察结果表明，位于内吞小泡中的二氧化硅颗粒的尺寸显著减小。通过监测含有胶体和可溶性硅物种的细胞培养基来确认溶解过程。这种溶解可以仅基于二氧化硅的溶解度来解释，并且对将非多孔二氧化硅颗粒用作细胞内药物释放系统具有重要意义。
