Schoenau E, Herzog K H, Michalk D
Klinik für Kinder und Jugendliche der Friedrich-Alexander Universität, Erlangen-Nürnberg, F.R.G.
Clin Chem. 1990 Nov;36(11):1934-6.
We detected in urine by HPLC two enzyme fractions of alkaline phosphatase (AP, EC 3.1.3.1), soluble and particulate, analogous to those in serum. The second fraction was eluted with high-salt-content eluent at the same elution time as high-molecular-mass, or particulate, AP in serum. AP characterization in urine from a patient with acute rejection crisis showed a greater sensitivity of the particulate form to treatment with L-phenylalanine, which suggests a higher content of intestinal-type AP in the particulate form. The soluble fraction showed a more liver-type AP behavior. Changes in the chromatograms after the sample was treated with 1-butanol and Triton X-100 support a membrane origin of the particulate AP. Urinalyses from patients with acute renal disease showed increased activity of soluble and particulate AP, with a relatively greater increase of particulate AP.
我们通过高效液相色谱法在尿液中检测到了碱性磷酸酶（AP，EC 3.1.3.1）的两种酶组分，即可溶性和颗粒性组分，与血清中的类似。第二个组分在与血清中高分子量或颗粒性AP相同的洗脱时间，用高盐含量洗脱剂洗脱。对一名急性排斥危机患者尿液中的AP进行表征发现，颗粒形式对L-苯丙氨酸处理的敏感性更高，这表明颗粒形式中肠型AP的含量更高。可溶性组分表现出更类似肝型AP的行为。样品用正丁醇和吐温X-100处理后色谱图的变化支持颗粒性AP的膜起源。急性肾病患者的尿液分析显示可溶性和颗粒性AP的活性增加，颗粒性AP的增加相对更大。
