Ketchum C H, Robinson C A, Huang S T
Department of Pathology, University of Alabama, Birmingham 35294.
Clin Chem. 1990 Nov;36(11):1956-7.
We report a simple and rapid procedure for assaying 8-methoxypsoralen (8-MOP) in plasma by high-performance liquid chromatography (HPLC). The standard curve for the assay is linear for 8-MOP from 15 to 500 micrograms/L (y = 0.002x-0.01, r = 0.99) with a lower limit of detection of 1.5 micrograms/L. Intra-assay precision (CV) was 6.0% at the 100 micrograms/L concentration and 10.0% at 50 micrograms/L (n = 30 each). Interassay precision was 6.4% at 100 micrograms/L and 7.0% at 50 micrograms/L (n = 50 each). Extraction recovery of 8-MOP was 98%. Common antiarrhythmics, sedatives, and hypnotics were found not to interfere.
我们报告了一种通过高效液相色谱法（HPLC）测定血浆中8-甲氧基补骨脂素（8-MOP）的简单快速方法。该测定的标准曲线在8-MOP浓度为15至500微克/升时呈线性（y = 0.002x - 0.01，r = 0.99），检测下限为1.5微克/升。在100微克/升浓度下，批内精密度（CV）为6.0%，在50微克/升时为10.0%（每组n = 30）。批间精密度在100微克/升时为6.4%，在50微克/升时为7.0%（每组n = 50）。8-MOP的提取回收率为98%。发现常见的抗心律失常药、镇静剂和催眠药不会产生干扰。
