皮层电刺激治疗减轻氯化铁诱导的癫痫大鼠的癫痫发作和行为迟缓
Alleviation of ferric chloride-induced seizures and retarded behaviour in epileptic rats by cortical electrical stimulation treatment.
作者信息
Wang C, Wu H, He F, Jing X, Liang Q, Heng G, Wang L, Gao G, Zhang H
机构信息
Department of Neurosurgery, Tangdu Hospital, and Department of Biomedical Engineering, Fourth Military Medical University, ShanXi, Xi'an, China.
出版信息
J Int Med Res. 2012;40(1):266-81. doi: 10.1177/147323001204000127.
OBJECTIVE
To study the effects of low-frequency cortical electrical stimulation (CES) on seizures and behaviour in a rat model of epilepsy induced by ferric chloride (FeCl3).
METHODS
Rats were randomly assigned into four groups (n=8 per group): normal healthy rats; saline-treated control rats; FeCl3-induced epileptic rats; CES-treated FeCl3-induced epileptic rats. Behavioural tests, analysis of the levels of brain-derived neurotrophic factor (BDNF) protein in brain tissue, and ultrastructural studies using transmission electron microscopy (TEM) were undertaken.
RESULTS
CES significantly decreased the number and grade of seizures, and improved rat behaviour, compared with untreated epileptic rats. CES reduced levels of BDNF protein in the forebrain and increased levels of BDNF protein in the hippocampus compared with untreated epileptic rats. TEM showed less damage and ultrastructural changes in the neurons of CES-treated epileptic rats.
CONCLUSIONS
CES inhibited seizures in FeCl3-induced epileptic rats and improved their behaviour. These effects might be mediated by altering BDNF protein levels in the brain.
目的
研究低频皮层电刺激（CES）对氯化铁（FeCl3）诱导的癫痫大鼠模型癫痫发作及行为的影响。
方法
将大鼠随机分为四组（每组n = 8只）：正常健康大鼠；生理盐水处理的对照大鼠；FeCl3诱导的癫痫大鼠；CES处理的FeCl3诱导的癫痫大鼠。进行行为测试、脑组织中脑源性神经营养因子（BDNF）蛋白水平分析以及使用透射电子显微镜（TEM）的超微结构研究。
结果
与未治疗的癫痫大鼠相比，CES显著降低了癫痫发作的次数和等级，并改善了大鼠行为。与未治疗的癫痫大鼠相比，CES降低了前脑BDNF蛋白水平，增加了海马体中BDNF蛋白水平。TEM显示，CES处理的癫痫大鼠神经元损伤和超微结构变化较少。
结论
CES抑制FeCl3诱导的癫痫大鼠的癫痫发作并改善其行为。这些作用可能是通过改变大脑中BDNF蛋白水平介导的。
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