人激肽释放酶 11（KLK11）mRNA 表达水平对喉癌患者的诊断和预后意义。
Diagnostic and prognostic significance of human kallikrein 11 (KLK11) mRNA expression levels in patients with laryngeal cancer.
机构信息
Department of Biochemistry and Molecular Biology, Faculty of Biology, University of Athens, 15701 Athens, Greece.
出版信息
Clin Biochem. 2012 Jun;45(9):623-30. doi: 10.1016/j.clinbiochem.2012.03.005. Epub 2012 Mar 11.
OBJECTIVES
Human kallikrein 11 gene (KLK11) encodes a secreted serine protease. In view of its diagnostic and prognostic strength in many malignancies, we investigated the mRNA expression levels of KLK11 in laryngeal tissues in order to unveil its clinical usefulness in laryngeal cancer.
DESIGN AND METHODS
KLK11 expression was quantified in 163 tissue samples from 105 laryngeal cancer patients with the development of a highly sensitive real-time PCR methodology, using SYBR Green® chemistry.
RESULTS
KLK11 expression in laryngeal cancer specimens of primary or recurrent nature was significantly inferior compared with their non-malignant counterparts (P<0.001 and P=0.026, respectively), a finding of immense diagnostic value as illustrated in the ROC curve analyses (P<0.001). Survival analysis showed that patients harboring KLK11-positive tumors had a significantly decreased risk of death (HR=0.26, P=0.042).
CONCLUSIONS
Our data recommend KLK11 mRNA expression as a novel and independent biomarker in laryngeal cancer for diagnostic and prognostic purposes.
目的
人激肽释放酶 11 基因（KLK11）编码一种分泌型丝氨酸蛋白酶。鉴于其在许多恶性肿瘤中的诊断和预后能力，我们研究了 KLK11 在喉组织中的 mRNA 表达水平，以期揭示其在喉癌中的临床应用价值。
设计和方法
采用 SYBR Green®化学法，利用高度敏感的实时 PCR 方法，对 105 例喉癌患者的 163 份组织样本中的 KLK11 表达进行了定量。
结果
原发性或复发性喉癌标本中的 KLK11 表达明显低于相应的非恶性对照（P<0.001 和 P=0.026），ROC 曲线分析显示这一发现具有巨大的诊断价值（P<0.001）。生存分析表明，携带 KLK11 阳性肿瘤的患者死亡风险显著降低（HR=0.26，P=0.042）。
结论
我们的数据推荐 KLK11 mRNA 表达作为喉癌的一种新的独立诊断和预后生物标志物。
Zotero 插件