OMIP-008：人类 T 细胞 Th1 和 Th2 细胞因子多功能性的测量。
OMIP-008: measurement of Th1 and Th2 cytokine polyfunctionality of human T cells.
机构信息
University of Wisconsin Carbone Cancer Center, Madison, Wisconsin, USA.
出版信息
Cytometry A. 2012 Jun;81(6):450-2. doi: 10.1002/cyto.a.22035. Epub 2012 Mar 19.
Abstract
Purpose and Appropriate Sample Types This panel was optimized to assess CD4 and CD8 T cell responses to various tumor antigens from melanoma patients. The panel was tested on single-cell derived T cell isolates (SCD-T) and T cell lines derived from peripheral blood mononuclear cells (PBMC) from melanoma patients, T cell lines from the tumor environment of melanoma patients, and fresh and cryopreserved PBMC (healthy donors). Staining can be performed in 96-well plates for high-throughput.
摘要
目的和适当的样本类型 该面板经过优化，可用于评估黑色素瘤患者对各种肿瘤抗原的 CD4 和 CD8 T 细胞反应。该面板已在源自黑色素瘤患者的单细胞衍生 T 细胞分离物 (SCD-T) 和外周血单核细胞 (PBMC) 衍生的 T 细胞系、源自黑色素瘤患者肿瘤环境的 T 细胞系以及新鲜和冷冻 PBMC（健康供体）上进行了测试。可以在 96 孔板中进行高通量染色。
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