Cournil I, Meyrand P, Moulins M
Laboratoire de Neurobiologie et Physiologie Comparées, Université de Bordeaux 1 et CNRS, Arcachon, France.
J Neurocytol. 1990 Aug;19(4):478-93. doi: 10.1007/BF01257238.
The stomatogastric ganglion of lobsters (Homarus or Jasus) contains a large number of gamma-aminobutyric acid-immunoreactive processes originating from ten fibres in the single input nerve, the stomatogastric nerve. The cell bodies and axonal pathways of these ten fibres have been identified using gamma-aminobutyric acid immunohistochemistry in combination with Lucifer Yellow staining (double labelling) and nickel chloride backfilling (selective gamma-aminobutyric acid immunoinhibition). It is shown that eight gamma-aminobutyric acid-immunoreactive neurons project to the stomatogastric ganglion: gamma-aminobutyric acid neurons 1 and 2, found posterior to the oesophageal ganglion, entering the stomatogastric nerve via the oesophageal nerve as well as sending an axonal branch into each superior oesophageal nerve; gamma-aminobutyric acid neurons 3 and 4, found anterior to the oesophageal ganglion, each sending an axonal branch into each inferior oesophageal nerve to reach the stomatogastric nerve via the commissural ganglion and the superior oesophageal nerve; and gamma-aminobutyric acid neurons 5 and 6, found in each commissural ganglion, projecting into the stomatogastric nerve via the inferior oesophageal nerve, the oesophageal ganglion and the oesophageal nerve. These gamma-aminobutyric acid-immunoreactive neurons were also characterized by electrophysiological methods coupled with Lucifer Yellow labelling, and their picrotoxin-sensitive effects on several stomatogastric ganglion neurons were demonstrated. The present results provide a firm basis for further studies concerning the physiological significance of one class of neurochemically-defined input neurons to stomatogastric ganglion networks.
龙虾（螯龙虾属或岩龙虾属）的口胃神经节包含大量源自单根输入神经即口胃神经中10条纤维的γ-氨基丁酸免疫反应性神经纤维束。利用γ-氨基丁酸免疫组织化学结合荧光黄染色（双重标记）和氯化镍逆行填充（选择性γ-氨基丁酸免疫抑制）已确定了这10条纤维的细胞体和轴突通路。结果表明，8个γ-氨基丁酸免疫反应性神经元投射至口胃神经节：γ-氨基丁酸神经元1和2位于食管神经节后方，经食管神经进入口胃神经，并向每条食管上神经发出一个轴突分支；γ-氨基丁酸神经元3和4位于食管神经节前方，各自向每条食管下神经发出一个轴突分支，经联合神经节和食管上神经到达口胃神经；γ-氨基丁酸神经元5和6位于每个联合神经节中，经食管下神经、食管神经节和食管神经投射至口胃神经。这些γ-氨基丁酸免疫反应性神经元也通过电生理方法结合荧光黄标记进行了表征，并证实了它们对几种口胃神经节神经元的荷包牡丹碱敏感性效应。目前的结果为进一步研究一类神经化学定义的输入神经元对口胃神经节网络的生理意义提供了坚实基础。
