Tsuchiya A, Sekikawa K, Ando Y, Suzuki S, Kimijima I, Abe R
Second Department of Surgery, Fukushima Medical College, Japan.
Jpn J Surg. 1990 Sep;20(5):510-4. doi: 10.1007/BF02471006.
Abnormal DNA content has been considered as an additional criterion for determining the biological behavior of a tumor. Flowcytometric DNA analysis was done on 121 patients with thyroid carcinoma encountered during the period between 1975 and 1987. Tumor tissues were sampled from paraffin-embedded blocks and the histology of thyroid carcinoma found to consist of 91 papillary, 23 follicular, 2 medullary, 1 squamous cell and 4 anaplastic carcinomas. The incidence of aneuploidy in thyroid carcinoma was 7.4 per cent (9 patients) while that of diploidy was 92.6 per cent (112 patients). The aneuploid specimens consisted of 6 papillary, 1 follicular, 1 medullary and 1 anaplastic carcinomas and, of 4 anaplastic carcinoma patients with subsequent death within 6 months, only 1 was aneuploid. As an indicator of proliferative potential, S-phase fraction (SPF) was also determined by flow cytometry, but this could not be used as an independent prognostic factor. The aneuploid patients showed a significantly decreased survival rate (p less than 0.01). Thus, although DNA measurement proved useful for predicting the survival of aneuploid patients, there is some discrepancy between DNA content and the biological behavior of the tumor.
异常的DNA含量已被视为确定肿瘤生物学行为的一项附加标准。对1975年至1987年期间收治的121例甲状腺癌患者进行了流式细胞术DNA分析。从石蜡包埋块中采集肿瘤组织，发现甲状腺癌的组织学类型包括91例乳头状癌、23例滤泡状癌、2例髓样癌、1例鳞状细胞癌和4例未分化癌。甲状腺癌中非整倍体的发生率为7.4%（9例患者），而二倍体的发生率为92.6%（112例患者）。非整倍体标本包括6例乳头状癌、1例滤泡状癌、1例髓样癌和1例未分化癌，在4例6个月内死亡的未分化癌患者中，只有1例是非整倍体。作为增殖潜能的指标，还通过流式细胞术测定了S期分数（SPF），但它不能用作独立的预后因素。非整倍体患者的生存率显著降低（p<0.01）。因此，虽然DNA检测被证明有助于预测非整倍体患者的生存情况，但DNA含量与肿瘤的生物学行为之间仍存在一些差异。
