Klemi P J, Joensuu H, Eerola E
Department of Pathology, University Central Hospital of Turku, Finland.
Am J Clin Pathol. 1988 Feb;89(2):154-9. doi: 10.1093/ajcp/89.2.154.
Nuclear DNA content of 19 cases of large cell or spindle cell anaplastic carcinomas of the thyroid was studied by flow cytometry. In 17 cases archival paraffin-embedded tissue and in 2 cases fresh material obtained by fine-needle aspiration biopsy was used for DNA content determination. Unequivocal evidence of DNA aneuploidy was obtained in 13 cases (68%). Two tumors contained more than one aneuploid stemline of cells. Eighteen (95%) of the patients died within 20 months after diagnosis, but 1 patient has survived for 13 years. The observed survival rate of the patients with carcinoma with a DNA index (DI) less than 1.30 was superior as compared with tumors with a DI greater than 1.30 (P = 0.007). All 7 patients with tumors with DIs greater than 1.30 died within seven months, whereas 5 of the 11 patients with tumors with DIs less than 1.30 survived longer than one year after the diagnosis.
通过流式细胞术研究了19例甲状腺大细胞或梭形细胞间变性癌的核DNA含量。17例使用存档石蜡包埋组织，2例使用细针穿刺活检获得的新鲜材料进行DNA含量测定。13例（68%）获得了明确的DNA非整倍体证据。两个肿瘤含有不止一个非整倍体细胞系。18例（95%）患者在诊断后20个月内死亡，但1例患者存活了13年。DNA指数（DI）小于1.30的癌患者的观察生存率优于DI大于1.30的肿瘤（P = 0.007）。DI大于1.30的所有7例患者在7个月内死亡，而DI小于1.30的11例患者中有5例在诊断后存活超过1年。
