Kang Jung Won, Koo Hye Cheong, Hwang Sun Young, Kang Sung Keun, Ra Jeong Chan, Lee Moon Han, Park Yong Ho
Department of Microbiology, College of Veterinary Medicine, and BK 21 Program for Veterinary Science, Seoul National University, Seoul 151-742, Korea.
J Vet Sci. 2012 Mar;13(1):23-31. doi: 10.4142/jvs.2012.13.1.23.
Human amniotic membrane-derived mesenchymal stem cells (hAM-MSCs) are capable of differentiating into several lineages and possess immunomodulatory properties. In this study, we investigated the soluble factor-mediated immunomodulatory effects of hAM-MSCs. Mitogen-induced peripheral blood mononuclear cell (PBMC) proliferation was suppressed by hAM-MSCs in a dose-dependent manner as well as hAM-MSC culture supernatant. Moreover, interferon-gamma and interleukin (IL)-17 production significantly decreased from PBMC, whereas IL-10 from PBMCs and transforming growth factor beta (TGF-β) production from hAM-MSCs significantly increased in co-cultures of hAM-MSCs and PBMCs. Production of several MSC factors, including hepatocyte growth factor (HGF), TGF-β, prostaglandin E2 (PGE2), and indoleamine 2, 3 dioxygenase (IDO), increased significantly in hAM-MSCs co-cultured with PBMCs. These results indicate that the immunomodulatory effects of hAM-MSCs may be associated with soluble factors (TGF-β, HGF, PGE2, and IDO), suggesting that hAM-MSCs may have potential clinical use in regenerative medicine.
人羊膜间充质干细胞（hAM-MSCs）能够分化为多个谱系，并具有免疫调节特性。在本研究中，我们调查了hAM-MSCs的可溶性因子介导的免疫调节作用。hAM-MSCs以及hAM-MSC培养上清液以剂量依赖的方式抑制有丝分裂原诱导的外周血单个核细胞（PBMC）增殖。此外，在hAM-MSCs与PBMC的共培养中，PBMC产生的干扰素-γ和白细胞介素（IL）-17显著减少，而PBMC产生的IL-10和hAM-MSCs产生的转化生长因子β（TGF-β）显著增加。在与PBMC共培养的hAM-MSCs中，包括肝细胞生长因子（HGF）、TGF-β、前列腺素E2（PGE2）和吲哚胺2,3-双加氧酶（IDO）在内的几种间充质干细胞因子的产生显著增加。这些结果表明，hAM-MSCs的免疫调节作用可能与可溶性因子（TGF-β、HGF、PGE2和IDO）有关，提示hAM-MSCs在再生医学中可能具有潜在的临床应用价值。
