Blanc Andrea Maria, Berois Mabel Beatriz, Tomé Lorena Magalí, Epstein Alberto L, Arbiza Juan Ramón
Sección Virología, Facultad de Ciencias, Universidad de la República, Montevideo 11600, Uruguay.
J Vet Sci. 2012 Mar;13(1):59-65. doi: 10.4142/jvs.2012.13.1.59.
Herpes simplex virus type-1 (HSV-1) amplicon vectors are versatile and useful tools for transferring genes into cells that are capable of stimulating a specific immune response to their expressed antigens. In this work, two HSV-1-derived amplicon vectors were generated. One of these expressed the full-length glycoprotein D (gD) of bovine herpesvirus 1 while the second expressed the truncated form of gD (gDtr) which lacked the trans-membrane region. After evaluating gD expression in the infected cells, the ability of both vectors to induce a specific gD immune response was tested in BALB/c mice that were intramuscularly immunized. Specific serum antibody responses were detected in mice inoculated with both vectors, and the response against truncated gD was higher than the response against full-length gD. These results reinforce previous findings that HSV-1 amplicon vectors can potentially deliver antigens to animals and highlight the prospective use of these vectors for treating infectious bovine rhinotracheitis disease.
1型单纯疱疹病毒（HSV-1）扩增载体是将基因导入能够对其表达的抗原产生特异性免疫反应的细胞的通用且有用的工具。在这项工作中，构建了两种源自HSV-1的扩增载体。其中一种表达牛疱疹病毒1型的全长糖蛋白D（gD），而另一种表达缺少跨膜区域的gD截短形式（gDtr）。在评估感染细胞中的gD表达后，在肌肉内免疫的BALB/c小鼠中测试了两种载体诱导特异性gD免疫反应的能力。在接种了两种载体的小鼠中均检测到特异性血清抗体反应，并且针对截短gD的反应高于针对全长gD的反应。这些结果强化了先前的发现，即HSV-1扩增载体可以潜在地将抗原递送至动物，并突出了这些载体在治疗传染性牛鼻气管炎疾病方面的潜在用途。
