The Vector-Borne Viral Diseases Programme, The Pirbright Institute, Pirbright, Woking, Surrey, United Kingdom.
PLoS One. 2013 Aug 27;8(8):e70779. doi: 10.1371/journal.pone.0070779. eCollection 2013.
The species Corriparta virus (CORV), within the genus Orbivirus, family Reoviridae, currently contains six virus strains: corriparta virus MRM1 (CORV-MRM1); CS0109; V654; V370; Acado virus and Jacareacanga virus. However, lack of neutralization assays, or reference genome sequence data has prevented further analysis of their intra-serogroup/species relationships and identification of individual serotypes. We report whole-genome sequence data for CORV-MRM1, which was isolated in 1960 in Australia. Comparisons of the conserved, polymerase (VP1), sub-core-shell 'T2' and core-surface 'T13' proteins encoded by genome segments 1, 2 and 8 (Seg-1, Seg-2 and Seg-8) respectively, show that this virus groups with the other mosquito borne orbiviruses. However, highest levels of nt/aa sequence identity (75.9%/91.6% in Seg-2/T2: 77.6%/91.7% in Seg-8/T13, respectively) were detected between CORV-MRM1 and California mosquito pool virus (CMPV), an orbivirus isolated in the USA in 1974, showing that they belong to the same virus species. The data presented here identify CMPV as a member of the Corriparta virus species and will facilitate identification of additional CORV isolates, diagnostic assay design and epidemiological studies.
科里帕塔病毒（CORV）属于呼肠孤病毒科呼肠孤病毒属，目前包含六种病毒株：MRM1 病毒（CORV-MRM1）、CS0109、V654、V370、Acado 病毒和雅卡里坎加病毒。然而，由于缺乏中和测定或参考基因组序列数据，无法进一步分析它们的血清型内种系关系和鉴定个别血清型。我们报告了 CORV-MRM1 的全基因组序列数据，该病毒于 1960 年在澳大利亚分离。对基因组片段 1、2 和 8（Seg-1、Seg-2 和 Seg-8）分别编码的保守聚合酶（VP1）、亚核心壳“T2”和核心表面“T13”蛋白进行比较，表明该病毒与其他蚊媒呼肠孤病毒聚集在一起。然而，在 Seg-2/T2（75.9%/91.6%）和 Seg-8/T13（77.6%/91.7%）中，CORV-MRM1 与 1974 年在美国分离的加利福尼亚蚊虫池病毒（CMPV）之间检测到最高水平的核苷酸/氨基酸序列同一性，表明它们属于同一病毒种。这里提供的数据将 CMPV 鉴定为科里帕塔病毒种的成员，将有助于鉴定其他 CORV 分离株、诊断检测设计和流行病学研究。
