da Silva Eduardo Caetano Brandão Ferreira, Samico Thammy Moura, Cardoso Rodrigo Rosa, Rabelo Marcelle Aquino, Bezerra Neto Armando Monteiro, de Melo Fábio Lopes, de Souza Lopes Ana Catarina, da Silva Aca Ivanize, Maciel Maria Amélia Vieira
Universidade Federal de Pernambuco, Recife, PE, Brasil.
Rev Esc Enferm USP. 2012 Feb;46(1):132-7. doi: 10.1590/s0080-62342012000100018.
This study was performed with the objective to identify the prevalence of colonization by Staphylococcus aureus in nursing professionals from a teaching hospital in Pernambuco, and evaluate the resistance profile of these isolates. To do this, we performed a cross-sectional study where biological samples were collected from the hands and nasal cavities of the subjects. S. aureus was identified using agar (blood agar and mannitol salt) via catalase and coagulase tests. The sensitivity profile was determined by Kirby Bauer technique and determination of methicillin resistance was performed with oxacillin screening with sodium chloride (NaCl) addition. Of the 151 professionals evaluated, 39 were colonized which showed a prevalence of 25.8%. Among the variables studied, age and use of PPE were associated with colonization by the organism. Of all the isolates, only five were resistant to methicillin.
本研究旨在确定伯南布哥州一家教学医院护理专业人员中金黄色葡萄球菌的定植率，并评估这些分离株的耐药情况。为此，我们开展了一项横断面研究，从受试者的手部和鼻腔采集生物样本。通过过氧化氢酶和凝固酶试验，使用琼脂（血琼脂和甘露醇盐琼脂）鉴定金黄色葡萄球菌。采用 Kirby Bauer 技术确定敏感性情况，并通过添加氯化钠（NaCl）的苯唑西林筛选法测定耐甲氧西林情况。在评估的 151 名专业人员中，39 人被定植，定植率为 25.8%。在所研究的变量中，年龄和个人防护装备的使用与该菌的定植有关。在所有分离株中，只有 5 株对甲氧西林耐药。
