Department of Clinical Laboratory, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, 510120, China.
Department of Clinical Laboratory, The Seventh Affiliated Hospital of Sun Yat-Sen University, Shenzhen, 518071, China.
BMC Infect Dis. 2018 Mar 12;18(1):122. doi: 10.1186/s12879-018-3024-x.
Medical laboratory staff are a high-risk population for colonization of Staphylococcus aureus (S. aureus) due to direct and dense contact with the pathogens; however, there is limited information about this colonization. This study sought to determine the prevalence and molecular characteristics of nasal colonization by S. aureus in medical laboratory staff in Guangzhou, southern China, and to compare the differences between microbiological laboratory (MLS) and non-microbiological laboratory (NMLS) staff.
S. aureus colonization was assessed by nasal swab cultures from 434 subjects, including 130 MLSs and 304 NMLSs from 33 hospitals in Guangzhou. All S. aureus isolates underwent the antimicrobial susceptibility test, virulence gene detection and molecular typing.
The overall prevalence of S. aureus carriage was 20.1% (87/434), which was higher in MLSs than in NMLSs (26.2% vs. 17.4%, P < 0.05), while the prevalence of Methicillin-resistant S. aureus (MRSA) was similar. Living with hospital staff was associated with S. aureus carriage. The majority of the isolates harboured various virulence genes, and those in MLSs appeared less resistant to antibiotics and more virulent than their counterparts. A total of 37 different spa types were detected; among these, t338, t437, t189 and t701 were the most frequently encountered types. T338 was the main spa type contributing to nasal colonization Methicillin-sensitive S. aureus (MSSA) (13.0%), and t437-SCCmec IV was predominant in MRSA isolates (40%).
These findings provide insight into the risk factors, molecular epidemiology and virulence gene profiles of S. aureus nasal carriage among the medical laboratory staff in Guangzhou.
由于与病原体的直接和密集接触，医学实验室工作人员是金黄色葡萄球菌（S. aureus）定植的高风险人群；然而，关于这种定植的信息有限。本研究旨在确定中国南方广州地区医学实验室工作人员鼻腔金黄色葡萄球菌定植的流行率和分子特征，并比较微生物实验室（MLS）和非微生物实验室（NMLS）工作人员之间的差异。
对来自广州 33 家医院的 434 名受试者进行鼻拭子培养，评估 S. aureus 定植情况。其中包括 130 名 MLS 和 304 名 NMLS。对所有 S. aureus 分离株进行了药敏试验、毒力基因检测和分子分型。
金黄色葡萄球菌携带率为 20.1%（87/434），MLS 中高于 NMLS（26.2% vs. 17.4%，P<0.05），耐甲氧西林金黄色葡萄球菌（MRSA）的流行率相似。与医院工作人员同住与金黄色葡萄球菌携带有关。大多数分离株携带各种毒力基因，而 MLS 中的分离株对抗生素的耐药性较低，毒力较强。共检测到 37 种不同的 spa 型；其中 t338、t437、t189 和 t701 是最常见的类型。t338 是导致鼻腔定植甲氧西林敏感金黄色葡萄球菌（MSSA）（13.0%）的主要 spa 型，而 t437-SCCmec IV 在 MRSA 分离株中占主导地位（40%）。
这些发现为广州地区医学实验室工作人员金黄色葡萄球菌鼻腔定植的危险因素、分子流行病学和毒力基因谱提供了深入了解。
