Li T-J, Dai Q-Z, Yin Y-L, Zhang J, Huang R-L, Ruan Z, Deng Z, Xie M
1Key Laboratory of Animal Nutrition and Health and Key Laboratory of Subtropical Agro-ecology, Institute of Subtropical Agriculture, The Chinese Academy of Sciences, PO Box 10, Changsha, Hunan 410125, PR China.
Animal. 2008 May;2(5):723-9. doi: 10.1017/S1751731108001614.
Four male pigs (Duroc × Landrace × Yorkshire; average initial (mean ± SEM) BW = 22.5 ± 1.1 kg), fitted with permanent catheters in the portal vein, ileal vein and carotid artery, were used in a 4 × 4 Latin square experimental design to measure the effect of dietary starch sources on the net portal appearance of glucose and amino acids. Dietary starch sources were resistant starch (RS), maize, sticky rice and brown rice. Diets were provided at 0730, 1530 and 2330 h during a 6-day adjustment period and 1-day collection period. On day 7 of each period, blood samples were collected from the portal vein and carotid artery at 0730 h (prior to feeding) and hourly up to 8 h after meal. Blood samples were used to determine glucose, amino acid, packed cell volume and partial pressure of oxygen (pO2). When calculated per 100 g feed intake, cumulative portal glucose appearance was lower (P < 0.05) for resistant starch than for maize, sticky rice or brown rice up to 8 h after the meal. Cumulative portal glucose appearance was higher (P < 0.05) for sticky rice and brown rice than for other diets until 4 h after the meal, but maize had higher cumulative glucose appearance after 4 h. Net cumulative portal concentrations of most amino acids for resistant starch were also reduced (P < 0.05) than for the other starch sources. Cumulative portal appearance of amino acid represented 48.39%, 63.76%, 61.80% and 59.18% of dietary intake for resistant starch, maize, sticky rice and brown rice, respectively. Collectively, our results indicate that dietary starch sources substantially affect the appearance of amino acids and glucose in the portal circulation.
选用4头雄性猪（杜洛克×长白×约克夏；初始平均体重（均值±标准误）=22.5±1.1千克），在门静脉、回肠静脉和颈动脉中植入永久性导管，采用4×4拉丁方实验设计，以测定日粮淀粉来源对门静脉葡萄糖和氨基酸净出现量的影响。日粮淀粉来源为抗性淀粉（RS）、玉米、糯米和糙米。在6天的适应期和1天的收集期内，于0730、1530和2330时提供日粮。在每个周期的第7天，于0730时（喂食前）从门静脉和颈动脉采集血样，并在进食后每小时采集一次，直至8小时。血样用于测定葡萄糖、氨基酸、红细胞压积和氧分压（pO2）。按每100克采食量计算，餐后8小时内，抗性淀粉组的门静脉葡萄糖累积出现量低于玉米、糯米或糙米组（P<0.05）。糯米和糙米组餐后4小时内的门静脉葡萄糖累积出现量高于其他日粮组（P<0.05），但4小时后玉米组的葡萄糖累积出现量更高。抗性淀粉组大多数氨基酸的门静脉净累积浓度也低于其他淀粉来源组（P<0.05）。抗性淀粉、玉米、糯米和糙米组氨基酸的门静脉累积出现量分别占日粮摄入量的48.39%、63.76%、61.80%和59.18%。总体而言，我们的结果表明，日粮淀粉来源对门静脉循环中氨基酸和葡萄糖的出现量有显著影响。
