Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, Seoul, Korea.
Theriogenology. 2012 Jul 1;78(1):110-5. doi: 10.1016/j.theriogenology.2012.01.026. Epub 2012 Mar 22.
The aim of this study was to assess the effects of trace mineral supplements near the time of ovulation on the number of ovulated oocytes, in vivo oocyte maturation and pregnancy for dog cloning. Sixteen oocyte donor dogs were used in each control and mineral supplement group, and 136 and 166 corpora lutea were counted from each group. No significant difference was observed between oocyte recovery rates in the control (91.2 ± 2.7%) and mineral (89.9 ± 2.7) groups. Proportions of mature (86.2 ± 7.2 and 88.4 ± 6.8%) and aged (13.8 ± 7.2 and 11.6 ± 6.8%) oocytes were not different in the control and mineral groups, respectively. Oocytes with fair (91.5 ± 3.6 and 93.6 ± 2.1%) and poor (8.5 ± 3.6 and 6.4 ± 2.1%) quality also showed no difference between the control and mineral groups. The concentrations of manganese and ferrous iron were higher and lower on the day of ovulation, respectively, in both groups, but trace element concentrations in peripheral blood were not affected by mineral treatment. Oocytes were used to make cloned embryos; after embryo transfer, four and two pups were delivered from the control and mineral group, respectively, but there was no difference in the delivery rate (4.6 and 2.7%). In conclusion, intravenous mineral supplements administered once close to the LH surge in oocyte donor dogs and recipients had no effect on the number of ovulated oocytes, in vivo oocyte maturation or pregnancy in dog cloning in this study.
本研究旨在评估在排卵临近时补充痕量矿物质对犬克隆中排卵卵母细胞数量、体内卵母细胞成熟和妊娠的影响。每个对照组和矿物质补充组使用了 16 只卵母细胞供体犬，每组分别计数了 136 和 166 个黄体。对照组（91.2±2.7%）和矿物质组（89.9±2.7%）的卵母细胞回收率无显著差异。成熟（86.2±7.2%和 88.4±6.8%）和老化（13.8±7.2%和 11.6±6.8%）卵母细胞的比例在对照组和矿物质组中也没有差异。质量良好（91.5±3.6%和 93.6±2.1%）和质量较差（8.5±3.6%和 6.4±2.1%）的卵母细胞在对照组和矿物质组之间也没有差异。两组排卵日锰和亚铁的浓度分别升高和降低，但矿物质处理对外周血痕量元素浓度没有影响。卵母细胞用于制作克隆胚胎；胚胎移植后，对照组和矿物质组分别产下 4 只和 2 只幼犬，但分娩率无差异（4.6%和 2.7%）。总之，在本研究中，在排卵临近时向卵母细胞供体犬和受体犬静脉内补充矿物质一次，对排卵卵母细胞数量、体内卵母细胞成熟或犬克隆妊娠没有影响。
