Key Laboratory of Industrial Ecology and Environmental Engineering (Ministry of Education, China), School of Environmental Science and Technology, Dalian University of Technology, Dalian 116024, China.
Anal Chim Acta. 2012 Apr 20;723:83-7. doi: 10.1016/j.aca.2012.02.030. Epub 2012 Feb 24.
In this paper, a simple and sensitive approach for H5N1 DNA detection was described based on the fluorescence resonance energy transfer (FRET) from quantum dots (QDs) to carbon nanotubes (CNTs) in a QDs-ssDNA/oxCNTs system, in which the QDs (CdTe) modified with ssDNA were used as donors. In the initial stage, with the strong interaction between ssDNA and oxCNTs, QDs fluorescence was effectively quenched. Upon the recognition of the target, the effective competitive bindings of it to QDs-ssDNA occurred, which decreased the interactions between the QDs-ssDNA and oxCNTs, leading to the recovery of the QDs fluorescence. The recovered fluorescence of QDs was linearly proportional to the concentration of the target in the range of 0.01-20 μM with a detection limit of 9.39 nM. Moreover, even a single-base mismatched target with the same concentration of target DNA can only recover a limited low fluorescence of QDs, illustrating the good anti-interference performance of this QDs-ssDNA/oxCNTs system. This FRET platform in the QDs-ssDNA/oxCNTs system was facilitated to the simple, sensitive and quantitative detection of virus nucleic acids and could have a wide range of applications in molecular diagnosis.
本文描述了一种基于量子点（QDs）到碳纳米管（CNTs）的荧光共振能量转移（FRET）的简单、灵敏的 H5N1 DNA 检测方法，该方法在 QDs-ssDNA/oxCNTs 体系中，用 ssDNA 修饰的 QDs（CdTe）作为供体。在初始阶段，由于 ssDNA 与 oxCNTs 之间的强相互作用，QDs 的荧光被有效猝灭。在识别目标后，它与 QDs-ssDNA 发生有效的竞争性结合，减少了 QDs-ssDNA 与 oxCNTs 之间的相互作用，导致 QDs 荧光恢复。恢复的 QDs 荧光与目标的浓度在 0.01-20 μM 范围内呈线性关系，检测限为 9.39 nM。此外，即使是与目标 DNA 浓度相同的单个碱基错配目标，也只能恢复有限的低荧光 QDs，这说明了该 QDs-ssDNA/oxCNTs 体系具有良好的抗干扰性能。这种基于 QDs-ssDNA/oxCNTs 体系的 FRET 平台促进了病毒核酸的简单、灵敏和定量检测，并在分子诊断中有广泛的应用。
