姜黄素和槲皮素对链脲佐菌素诱导的糖尿病大鼠溶酶体酶活性的影响。

Effect of curcumin and quercetin on lysosomal enzyme activities in streptozotocin-induced diabetic rats.

机构信息

Dept. of Biochemistry and Nutrition, Central Food Technological Research Institute, CSIR, Mysore - 570 020, India.

出版信息

Clin Nutr. 2012 Oct;31(5):749-55. doi: 10.1016/j.clnu.2012.02.003. Epub 2012 Mar 24.

DOI:10.1016/j.clnu.2012.02.003

Abstract

BACKGROUND & AIMS: Diabetes causes impairment of various enzyme activities in the physiological system, including lysosomal enzymes. The effect of feeding curcumin, quercetin and aminoguanidine on lysosomal enzyme activities viz., N-acetyl-β-d-glucosaminidase, β-d-glucuronidase, β-d-galactosidase and acid phosphatase were studied in different tissues of streptozotocin-induced diabetic rats.

METHOD

Rats were divided into four control groups and four diabetic groups. Experimental groups were fed with diet supplemented with curcumin (0.5%) or quercetin (0.1%) or aminoguanidine (0.05%). Lysosomal enzyme activities were determined in various tissues.

RESULTS

The specific activity of N-acetyl-β-d-glucosaminidase in liver of diabetic rats was decreased when compared to control rats and was ameliorated with curcumin and quercetin treatment by 67% and 78%, respectively. On the other hand, β-d-glucuronidase activity was higher in the brain of diabetic rats (0.90 ± 0.04 nmol/mg protein/min), when compared to control rats (0.45 ± 0.02 nmol/mg protein/min) and was decreased in curcumin (0.75 ± 0.05 nmol/mg protein/min) and quercetin (0.74 ± 0.11 nmol/mg protein/min) treated rats. β-d-galactosidase activity in spleen of curcumin and quercetin fed diabetic group rats was ameliorated by 68% and 58%, respectively, in comparison to diabetic rats. Acid phosphatase activity in diabetic rats decreased in testis when compared to control.

CONCLUSION

Curcumin and quercetin feeding modulated lysosomal enzyme activities in different tissues during diabetes and the effect was comparable to well-known anti-glycative agent - aminoguanidine.

摘要

背景与目的

糖尿病会导致生理系统中各种酶活性的损伤，包括溶酶体酶。本研究旨在探讨姜黄素、槲皮素和氨基胍对链脲佐菌素诱导的糖尿病大鼠不同组织中溶酶体酶活性（N-乙酰-β-D-氨基葡萄糖苷酶、β-D-葡糖醛酸酶、β-D-半乳糖苷酶和酸性磷酸酶）的影响。

方法

将大鼠分为 4 个对照组和 4 个糖尿病组。实验组大鼠给予补充姜黄素（0.5%）或槲皮素（0.1%）或氨基胍（0.05%）的饮食。测定各种组织中的溶酶体酶活性。

结果

与对照组大鼠相比，糖尿病大鼠肝脏中 N-乙酰-β-D-氨基葡萄糖苷酶的比活性降低，而姜黄素和槲皮素处理可分别改善 67%和 78%。另一方面，糖尿病大鼠大脑中的β-D-葡糖醛酸酶活性较高（0.90±0.04 nmol/mg 蛋白/min），明显高于对照组（0.45±0.02 nmol/mg 蛋白/min），而姜黄素（0.75±0.05 nmol/mg 蛋白/min）和槲皮素（0.74±0.11 nmol/mg 蛋白/min）处理可降低该酶活性。与糖尿病大鼠相比，姜黄素和槲皮素喂养的糖尿病大鼠脾脏中的β-D-半乳糖苷酶活性分别改善了 68%和 58%。与对照组相比，糖尿病大鼠睾丸中的酸性磷酸酶活性降低。