College of Veterinary Medicine and Applied Radiological Science Institute, Jeju National University, Jeju, South Korea.
Food Chem Toxicol. 2012 Jun;50(6):2097-105. doi: 10.1016/j.fct.2012.03.041. Epub 2012 Mar 17.
Vanadium, an essential micronutrient, has been implicated in controlling diabetes and carcinogenesis and in impeding reactive oxygen species (ROS) generation. γ-ray irradiation triggers DNA damage by inducing ROS production and causes diminution in radiosensitive immunocytes. In this study, we elucidate the immune activation capacities of Jeju water containing vanadium on immunosuppression caused by γ-ray irradiation, and identify its mechanism using various low doses of NaVO(3). We examined the intracellular ROS generation, DNA damage, cell proliferation, population of splenocytes, and cytokine/antibody profiles in irradiated mice drinking Jeju water for 180 days and in non-irradiated and in irradiated splenocytes both of which were treated with NaVO(3). Both Jeju water and 0.245 μM NaVO(3) attenuated the intracellular ROS generation and DNA damage in splenocytes against γ-ray irradiation. Splenocytes were significantly proliferated by the long-term intake of Jeju water and by 0.245 μM NaVO(3) treatment, and the expansion of B cells accounted for the increased number of splenocytes. Also, 0.245 μM NaVO(3) treatment showed the potency to amplify the production of IFN-γ and total IgG in irradiated splenocytes, which correlated with the expansion of B cells. Collectively, Jeju water containing vanadium possesses the immune activation property against damages caused by γ-irradiation.
钒是一种必需的微量元素，它被认为可以控制糖尿病和癌症的发生，并抑制活性氧（ROS）的产生。γ 射线照射通过诱导 ROS 的产生来触发 DNA 损伤，并导致辐射敏感免疫细胞减少。在这项研究中，我们阐明了含有钒的济州水对 γ 射线照射引起的免疫抑制的免疫激活能力，并使用各种低剂量的 NaVO（3）确定了其机制。我们检查了饮用济州水 180 天的辐照小鼠以及用 NaVO（3）处理的未辐照和辐照脾细胞中的细胞内 ROS 生成、DNA 损伤、细胞增殖、脾细胞群体和细胞因子/抗体谱。济州水和 0.245μM NaVO（3）均减轻了 γ 射线照射对脾细胞内 ROS 生成和 DNA 损伤的影响。长期摄入济州水和 0.245μM NaVO（3）处理可显著促进脾细胞增殖，B 细胞的扩增导致脾细胞数量增加。此外，0.245μM NaVO（3）处理显示出在辐照脾细胞中放大 IFN-γ和总 IgG 产生的能力，这与 B 细胞的扩增相关。总的来说，含有钒的济州水具有针对 γ 射线照射引起的损伤的免疫激活特性。
