Ruhland G J, Fiedler F
Institut für Genetik und Mikrobiologie, Universität München, Federal Republic of Germany.
Arch Microbiol. 1990;154(4):375-9. doi: 10.1007/BF00276534.
Lipoteichoic acids were isolated from eleven species of the genus Staphylococcus using phenol-water partition and hydrophobic chromatography on octyl-Sepharose CL-4B. The lipoteichoic acids purified could be visualized by SDS-PAGE. They were shown to be composed of a hydrophilic poly(glycerophosphate) chain covalently linked to gentiobiosyldiacylglycerol, the common lipid anchor of these molecules. Glycerophosphate units of the hydrophilic chain were found to be partly substituted with ester-linked D-alanine, except in the case of S. cohnii. The lipoteichoic acids isolated from S. cohnii, S. hominis, S. saprophyticus and S. simulans contain alpha(1-2)-linked N-acetylglucosamine as an additional substituent of the poly(glycerophosphate) backbone.
利用酚-水分配法和辛基-琼脂糖CL-4B疏水色谱法，从11种葡萄球菌属菌株中分离出脂磷壁酸。纯化后的脂磷壁酸可通过SDS-PAGE进行可视化分析。结果表明，它们由一条亲水的聚（甘油磷酸）链共价连接到龙胆二糖二酰基甘油上组成，龙胆二糖二酰基甘油是这些分子常见的脂质锚定基团。除科氏葡萄球菌外，亲水链的甘油磷酸单元部分被酯键连接的D-丙氨酸取代。从科氏葡萄球菌、人葡萄球菌、腐生葡萄球菌和模仿葡萄球菌中分离出的脂磷壁酸，在聚（甘油磷酸）主链上含有α(1-2)-连接的N-乙酰葡糖胺作为额外的取代基。
