University of California Los Angeles School of Dentistry and Dental Research Institute, Los Angeles, California, United States of America.
PLoS One. 2012;7(3):e33037. doi: 10.1371/journal.pone.0033037. Epub 2012 Mar 20.
Saliva is a useful biofluid for the early detection of disease, but how distal tumors communicate with the oral cavity and create disease-specific salivary biomarkers remains unclear. Using an in vitro breast cancer model, we demonstrated that breast cancer-derived exosome-like microvesicles are capable of interacting with salivary gland cells, altering the composition of their secreted exosome-like microvesicles. We found that the salivary gland cells secreted exosome-like microvesicles encapsulating both protein and mRNA. We also showed that the interaction with breast cancer-derived exosome-like microvesicles communicated and activated the transcriptional machinery of the salivary gland cells. Thus, the interaction altered the composition of the salivary gland cell-derived exosome-like microvesicles on both the transcriptomically and proteomically.
唾液是一种用于疾病早期检测的有用生物体液，但远端肿瘤如何与口腔沟通并产生特定于疾病的唾液生物标志物尚不清楚。我们使用体外乳腺癌模型证明，乳腺癌来源的外泌体样微囊泡能够与唾液腺细胞相互作用，改变其分泌的外泌体样微囊泡的组成。我们发现唾液腺细胞分泌的外泌体样微囊泡包裹着蛋白质和 mRNA。我们还表明，与乳腺癌来源的外泌体样微囊泡的相互作用沟通并激活了唾液腺细胞的转录机制。因此，这种相互作用改变了唾液腺细胞来源的外泌体样微囊泡在转录组和蛋白质组层面上的组成。
