在作为异种移植物扩增的小鼠患者急性淋巴细胞白血病细胞中进行有效的 RNA 干扰。
Efficient RNA interference in patients' acute lymphoblastic leukemia cells amplified as xenografts in mice.
机构信息
Department of Gene Vectors, Helmholtz Center Munich - German Research Center for Environmental Health, Marchioninistr, 25, 81377 Munich, Germany.
出版信息
Cell Commun Signal. 2012 Mar 26;10(1):8. doi: 10.1186/1478-811X-10-8.
BACKGROUND
Signaling studies in cell lines are hampered by non-physiological alterations obtained in vitro. Physiologic primary tumor cells from patients with leukemia require passaging through immune-compromised mice for amplification. The aim was to enable molecular work in patients' ALL cells by establishing siRNA transfection into cells amplified in mice.
RESULTS
We established delivering siRNA into these cells without affecting cell viability. Knockdown of single or multiple genes reduced constitutive or induced protein expression accompanied by marked signaling alterations.
CONCLUSION
Our novel technique allows using patient-derived tumor cells instead of cell lines for signaling studies in leukemia.
背景
细胞系中的信号研究受到体外获得的非生理改变的阻碍。患有白血病的患者的生理原发性肿瘤细胞需要通过免疫功能低下的小鼠传代以扩增。目的是通过建立在小鼠中扩增的细胞中的 siRNA 转染，使患者 ALL 细胞中的分子工作成为可能。
结果
我们建立了将 siRNA 递送到这些细胞中的方法，而不会影响细胞活力。敲低单个或多个基因会降低组成性或诱导的蛋白表达，并伴有明显的信号改变。
结论
我们的新技术允许使用患者来源的肿瘤细胞代替白血病中的细胞系进行信号研究。
Zotero 插件