Baxter Healthcare Corporation, Round Lake, Illinois 60073, USA.
Anal Chem. 2012 May 1;84(9):4104-10. doi: 10.1021/ac300176z. Epub 2012 Apr 18.
Monosaccharide analysis is a critical way to profile the composition of complex carbohydrates. Methods to analyze neutral and amino sugars have been established for a long time, but methods for acidic sugars are rare. The acidic sugars, including uronic acids and sialic acids, are also important components in some complex carbohydrates. In this report, a high-performance anion-exchange chromatography method with pulsed amperometric detection was initially developed to analyze acidic sugars including different uronic acids and sialic acids. Subsequently, a method to profile complete monosaccharides, including most neutral, amino, and acidic sugars, was developed. This method has a limit of quantitation of ~12.5 × 10(-3) nmol for each sugar as well as good linearity over a wide range. This is a convenient procedure because it avoids additional derivatization of monosaccharides and has a broad application to a wide range of complex carbohydrates. The monosaccharide compositions of a variety of complex carbohydrates such as different glycosaminoglycans, alginate, fucoidan, and glycans were profiled by this comprehensive method. In addition, the hydrolysis patterns of these complex carbohydrates are discussed.
单糖分析是分析复杂碳水化合物组成的一种重要方法。分析中性和氨基糖的方法已经建立了很长时间，但分析酸性糖的方法却很少。酸性糖，包括糖醛酸和唾液酸，也是一些复杂碳水化合物的重要组成部分。在本报告中，我们最初开发了一种高效阴离子交换色谱法与脉冲安培检测法，用于分析包括不同糖醛酸和唾液酸在内的酸性糖。随后，我们开发了一种分析完整单糖的方法，包括大多数中性、氨基和酸性糖。该方法对每种糖的定量限约为 12.5×10(-3) nmol，并且在很宽的范围内具有良好的线性关系。由于该方法避免了对单糖的额外衍生化，因此非常方便，并且具有广泛的应用范围。该综合方法可用于分析多种复杂碳水化合物，如不同的糖胺聚糖、藻酸盐、褐藻糖胶和聚糖的单糖组成。此外，还讨论了这些复杂碳水化合物的水解模式。
