Department of Chemical and Biomolecular Engineering, University of Tennessee, Knoxville, TN 37996-2200, USA.
Arch Biochem Biophys. 2012 Oct 15;526(2):99-106. doi: 10.1016/j.abb.2012.03.009. Epub 2012 Mar 19.
Since its first application to antibody engineering 15 years ago, yeast display technology has been developed into a highly potent tool for both affinity maturing lead molecules and isolating novel antibodies and antibody-like species. Robust approaches to the creation of diversity, construction of yeast libraries, and library screening or selection have been elaborated, improving the quality of engineered molecules and certainty of success in an antibody engineering campaign and positioning yeast display as one of the premier antibody engineering technologies currently in use. Here, we summarize the history of antibody engineering by yeast surface display, approaches used in its application, and a number of examples highlighting the utility of this method for antibody engineering.
自 15 年前首次应用于抗体工程以来，酵母展示技术已发展成为一种非常有效的亲和力成熟先导分子的工具，同时也可用于分离新型抗体和抗体样物质。已经详细阐述了创建多样性、构建酵母文库以及文库筛选或选择的稳健方法，从而提高了工程分子的质量，并确保了抗体工程中的成功率，使酵母展示成为目前使用的主要抗体工程技术之一。在这里，我们总结了通过酵母表面展示进行抗体工程的历史、应用中使用的方法以及一些突出该方法在抗体工程中实用性的实例。
