• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

哺乳动物展示技术在治疗性抗体开发及其他领域的进展：现状、挑战与未来展望。

Advancements in mammalian display technology for therapeutic antibody development and beyond: current landscape, challenges, and future prospects.

机构信息

Discovery & Engineering Division, Iontas Ltd./FairJourney Biologics, Cambridge, United Kingdom.

Technology Division, Iontas/FairJourney Biologics, Cambridge, United Kingdom.

出版信息

Front Immunol. 2024 Sep 24;15:1469329. doi: 10.3389/fimmu.2024.1469329. eCollection 2024.

DOI:10.3389/fimmu.2024.1469329
PMID:39381002
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11459229/
Abstract

The evolving development landscape of biotherapeutics and their growing complexity from simple antibodies into bi- and multi-specific molecules necessitates sophisticated discovery and engineering platforms. This review focuses on mammalian display technology as a potential solution to the pressing challenges in biotherapeutic development. We provide a comparative analysis with established methodologies, highlighting key aspects of mammalian display technology, including genetic engineering, construction of display libraries, and its pivotal role in hit selection and/or developability engineering. The review delves into the mechanisms underpinning developability-driven selection via mammalian display and their broader implications. Applications beyond antibody discovery are also explored, alongside advancements towards function-first screening technologies, precision genome engineering and AI/ML-enhanced libraries, situating them in the context of mammalian display. Overall, the review provides a comprehensive overview of the current mammalian display technology landscape, underscores the expansive potential of the technology for biotherapeutic development, addresses the critical challenges for the full realisation of this potential, and examines advances in related disciplines that might impact the future application of mammalian display technologies.

摘要

生物疗法的不断发展的领域及其从简单抗体向双特异性和多特异性分子的日益复杂，需要复杂的发现和工程平台。本文重点介绍哺乳动物展示技术作为解决生物疗法开发中紧迫挑战的潜在解决方案。我们与已建立的方法进行了比较分析，突出了哺乳动物展示技术的关键方面，包括遗传工程、展示文库的构建，以及其在命中选择和/或可开发性工程中的关键作用。本文深入探讨了通过哺乳动物展示实现可开发性驱动选择的机制及其更广泛的影响。除了抗体发现之外，还探讨了该技术的其他应用，以及朝着功能优先筛选技术、精确基因组工程和 AI/ML 增强文库的进展，将它们置于哺乳动物展示的背景下。总的来说，本文全面概述了当前哺乳动物展示技术的现状，强调了该技术在生物疗法开发中的广泛潜力，解决了充分实现这一潜力的关键挑战，并探讨了相关学科的进展，这些进展可能会影响哺乳动物展示技术的未来应用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c5a6/11459229/fe2db8c2813c/fimmu-15-1469329-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c5a6/11459229/ade0976c915f/fimmu-15-1469329-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c5a6/11459229/6a44596c5bd1/fimmu-15-1469329-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c5a6/11459229/4f839ee44e95/fimmu-15-1469329-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c5a6/11459229/94626d35dc56/fimmu-15-1469329-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c5a6/11459229/1ba1048d9e84/fimmu-15-1469329-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c5a6/11459229/ce1a6ac0fffb/fimmu-15-1469329-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c5a6/11459229/2d7147215323/fimmu-15-1469329-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c5a6/11459229/fe2db8c2813c/fimmu-15-1469329-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c5a6/11459229/ade0976c915f/fimmu-15-1469329-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c5a6/11459229/6a44596c5bd1/fimmu-15-1469329-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c5a6/11459229/4f839ee44e95/fimmu-15-1469329-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c5a6/11459229/94626d35dc56/fimmu-15-1469329-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c5a6/11459229/1ba1048d9e84/fimmu-15-1469329-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c5a6/11459229/ce1a6ac0fffb/fimmu-15-1469329-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c5a6/11459229/2d7147215323/fimmu-15-1469329-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c5a6/11459229/fe2db8c2813c/fimmu-15-1469329-g008.jpg

相似文献

1
Advancements in mammalian display technology for therapeutic antibody development and beyond: current landscape, challenges, and future prospects.哺乳动物展示技术在治疗性抗体开发及其他领域的进展：现状、挑战与未来展望。
Front Immunol. 2024 Sep 24;15:1469329. doi: 10.3389/fimmu.2024.1469329. eCollection 2024.
2
Evolution of phage display libraries for therapeutic antibody discovery.噬菌体展示文库在治疗性抗体发现中的演变。
MAbs. 2023 Jan-Dec;15(1):2213793. doi: 10.1080/19420862.2023.2213793.
3
Phage Display Libraries for Antibody Therapeutic Discovery and Development.用于抗体治疗发现与开发的噬菌体展示文库
Antibodies (Basel). 2019 Aug 23;8(3):44. doi: 10.3390/antib8030044.
4
Transpo-mAb display: Transposition-mediated B cell display and functional screening of full-length IgG antibody libraries.转座介导的单克隆抗体展示：转座介导的全长IgG抗体文库的B细胞展示及功能筛选。
MAbs. 2016 May-Jun;8(4):726-40. doi: 10.1080/19420862.2016.1160990. Epub 2016 Mar 17.
5
Therapeutic Antibody Engineering and Selection Strategies.治疗性抗体工程与选择策略。
Adv Biochem Eng Biotechnol. 2020;171:55-86. doi: 10.1007/10_2019_116.
6
Phage Display: A promising selection strategy for the improvement of antibody targeting and drug delivery properties.噬菌体展示：一种用于改善抗体靶向性和药物递送特性的有前景的筛选策略。
Front Microbiol. 2022 Sep 26;13:962124. doi: 10.3389/fmicb.2022.962124. eCollection 2022.
7
Beyond affinity: selection of antibody variants with optimal biophysical properties and reduced immunogenicity from mammalian display libraries.超越亲和力：从哺乳动物展示文库中选择具有最佳物理特性和降低免疫原性的抗体变体。
MAbs. 2020 Jan-Dec;12(1):1829335. doi: 10.1080/19420862.2020.1829335.
8
Eukaryotic ribosome display for antibody discovery: A review.真核核糖体展示技术在抗体发现中的应用：综述
Hum Antibodies. 2024;32(3):107-120. doi: 10.3233/HAB-240001.
9
Phage display-derived human antibodies in clinical development and therapy.临床开发与治疗中基于噬菌体展示技术获得的人源抗体。
MAbs. 2016 Oct;8(7):1177-1194. doi: 10.1080/19420862.2016.1212149. Epub 2016 Jul 14.
10
Engineering antibodies by yeast display.通过酵母展示工程化抗体。
Arch Biochem Biophys. 2012 Oct 15;526(2):99-106. doi: 10.1016/j.abb.2012.03.009. Epub 2012 Mar 19.

引用本文的文献

1
Recent Advances in Antibody Discovery Using Ultrahigh-Throughput Droplet Microfluidics: Challenges and Future Perspectives.利用超高通量微滴微流控技术进行抗体发现的最新进展：挑战与未来展望
Biosensors (Basel). 2025 Jun 25;15(7):409. doi: 10.3390/bios15070409.
2
Isolation of a Monoclonal Human scFv Against Cytomegalovirus pp71 Antigen Using Yeast Display.利用酵母展示技术分离抗巨细胞病毒pp71抗原的单克隆人单链抗体片段
Antibodies (Basel). 2025 Jul 10;14(3):57. doi: 10.3390/antib14030057.
3
A Targeted Integration-Based CHO Cell Platform for Simultaneous Antibody Display and Secretion.

本文引用的文献

1
De novo generation of SARS-CoV-2 antibody CDRH3 with a pre-trained generative large language model.从头生成 SARS-CoV-2 抗体 CDRH3 与预训练生成式大型语言模型。
Nat Commun. 2024 Aug 10;15(1):6867. doi: 10.1038/s41467-024-50903-y.
2
Biophysical cartography of the native and human-engineered antibody landscapes quantifies the plasticity of antibody developability.天然和人工改造抗体景观的生物物理作图定量评估了抗体可开发性的可塑性。
Commun Biol. 2024 Jul 31;7(1):922. doi: 10.1038/s42003-024-06561-3.
3
A comprehensive overview of recent advances in generative models for antibodies.
一种基于靶向整合的CHO细胞平台，用于同时进行抗体展示和分泌。
Antibodies (Basel). 2025 Apr 28;14(2):38. doi: 10.3390/antib14020038.
抗体生成模型近期进展的全面概述。
Comput Struct Biotechnol J. 2024 Jun 20;23:2648-2660. doi: 10.1016/j.csbj.2024.06.016. eCollection 2024 Dec.
4
The new frontier in CHO cell line development: From random to targeted transgene integration technologies.CHO 细胞系开发的新前沿：从随机到靶向转基因整合技术。
Biotechnol Adv. 2024 Oct;75:108402. doi: 10.1016/j.biotechadv.2024.108402. Epub 2024 Jun 29.
5
Increased stable integration efficiency in CHO cells through enhanced nuclear localization of Bxb1 serine integrase.通过增强 Bxb1 丝氨酸整合酶的核定位，提高 CHO 细胞的稳定整合效率。
BMC Biotechnol. 2024 Jun 26;24(1):44. doi: 10.1186/s12896-024-00871-4.
6
Context-dependent genomic locus effects on antibody production in recombinant Chinese hamster ovary cells generated through random integration.通过随机整合产生的重组中国仓鼠卵巢细胞中，依赖于上下文的基因组位点对抗体产生的影响。
Comput Struct Biotechnol J. 2024 Apr 10;23:1654-1665. doi: 10.1016/j.csbj.2024.04.023. eCollection 2024 Dec.
7
Fast-Track Discovery of SARS-CoV-2-Neutralizing Antibodies from Human B Cells by Direct Functional Screening.直接功能筛选快速发现针对 SARS-CoV-2 的中和抗体的人 B 细胞。
Viruses. 2024 Feb 22;16(3):339. doi: 10.3390/v16030339.
8
Rapid isolation of pan-neutralizing antibodies against Omicron variants from convalescent individuals infected with SARS-CoV-2.从感染 SARS-CoV-2 的康复个体中快速分离出针对奥密克戎变异株的pan 中和抗体。
Front Immunol. 2024 Mar 6;15:1374913. doi: 10.3389/fimmu.2024.1374913. eCollection 2024.
9
The rise of big data: deep sequencing-driven computational methods are transforming the landscape of synthetic antibody design.大数据的兴起：深度测序驱动的计算方法正在改变合成抗体设计的格局。
J Biomed Sci. 2024 Mar 16;31(1):29. doi: 10.1186/s12929-024-01018-5.
10
A pivotal decade for bispecific antibodies?双特异性抗体的关键十年？
MAbs. 2024 Jan-Dec;16(1):2321635. doi: 10.1080/19420862.2024.2321635. Epub 2024 Mar 11.