Key Laboratory of Food Safety of Zhejiang Province, Food Quality and Safety Department, Zhejiang Gongshang University, 149 Jiaogong Road, Hangzhou 310035 Zhejiang province, China.
Biol Trace Elem Res. 2012 Oct;149(1):110-6. doi: 10.1007/s12011-012-9395-8. Epub 2012 Mar 28.
The experiment was designed to investigate the effect of selenium (Se) chemical forms (sodium selenite, selenium nanoparticle [nano-Se] and selenomethionine) on the transport, uptake and glutathione peroxidase (GSH-Px) activity in the Caco-2 cell model. The transport and uptake of different forms of Se (0.1 μmol l(-1)) across the Caco-2 cell monolayer were carried out in two directions (apical [AP] to basolateral [BL] and BL to AP) for 2 h, respectively, and the apparent permeability coefficient (P(app)), transport efficiency and uptake efficiency were all calculated. In the present study, the transport and uptake of three forms of Se were time-dependent both in AP to BL and BL to AP directions. By the end of 2 h, the transport efficiencies of selenomethionine and nano-Se were higher than that of sodium selenite (P<0.05). The highest uptake efficiency (P<0.05) was observed in cells treated with nano-Se and significant difference (P<0.05) was also observed between the cells incubated with sodium selenite and selenomethionine. As for the P(app), sodium selenite (P<0.05) had the lowest values compared with that of selenomethionine and nano-Se, in both AP-BL and BL-AP. However, no significant differences were observed in GSH-Px activities. These results indicated that the efficiency of Se in the Caco-2 cells varied with its chemical forms, which might be associated with the differences in Se transport and uptake.
本实验旨在研究硒（Se）化学形式（亚硒酸钠、硒纳米粒子[nano-Se]和硒代蛋氨酸）对 Caco-2 细胞模型中硒的转运、摄取和谷胱甘肽过氧化物酶（GSH-Px）活性的影响。分别在两个方向（AP 到 BL 和 BL 到 AP）进行 2 小时的不同形式 Se（0.1 μmol l(-1))的跨 Caco-2 细胞单层转运和摄取实验，并计算表观渗透系数（P(app)）、转运效率和摄取效率。在本研究中，三种形式的 Se 在 AP 到 BL 和 BL 到 AP 方向的转运和摄取均呈时间依赖性。在 2 小时结束时，硒代蛋氨酸和纳米硒的转运效率高于亚硒酸钠（P<0.05）。纳米硒处理组的摄取效率最高（P<0.05），且与亚硒酸钠和硒代蛋氨酸孵育的细胞之间也存在显著差异（P<0.05）。对于 P(app)，亚硒酸钠（P<0.05）在 AP-BL 和 BL-AP 中均低于硒代蛋氨酸和纳米硒。然而，GSH-Px 活性无显著差异。这些结果表明，Caco-2 细胞中 Se 的效率因其化学形式而异，这可能与 Se 的转运和摄取差异有关。
