Mizuno Masayuki, Yurimoto Hiroya, Yoshida Naoko, Iguchi Hiroyuki, Sakai Yasuyoshi
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto, Japan.
Biosci Biotechnol Biochem. 2012;76(3):578-80. doi: 10.1271/bbb.110737.
The distribution of pink-pigmented facultative methylotrophs (PPFMs) on the leaves of various vegetables was studied. All kinds of vegetable leaves tested gave pink-pigmented colonies on agar plates containing methanol as sole carbon source. The numbers of PPFMs on the leaves, colony-forming units (CFU)/g of fresh leaves, differed among the plants, although they were planted and grown at the same farm. Commercial green perilla, Perilla frutescens viridis (Makino) Makino, gave the highest counts of PPFMs (2.0-4.1×10(7) CFU/g) of all the commercial vegetable leaves tested, amounting to 15% of total microbes on the leaves. The PPFMs isolated from seeds of two varieties of perilla, the red and green varieties, exhibited high sequence similarity as to the 16S rRNA gene to two different Methylobacterium species, M. fujisawaense DSM5686(T) and M. radiotolerans JCM2831(T) respectively, suggesting that there is specific interaction between perilla and the PPFMs.
研究了不同蔬菜叶片上粉红色兼性甲基营养菌（PPFMs）的分布情况。在以甲醇作为唯一碳源的琼脂平板上，所有测试的蔬菜叶片均长出了粉红色菌落。尽管这些蔬菜是在同一个农场种植和生长的，但不同植株叶片上PPFMs的数量，即每克鲜叶的菌落形成单位（CFU）有所不同。在所有测试的市售蔬菜叶片中，市售青紫苏（Perilla frutescens viridis (Makino) Makino）叶片上的PPFMs数量最多（2.0 - 4.1×10⁷ CFU/g），占叶片上微生物总数的15%。从紫苏的两个品种（红色和绿色）种子中分离出的PPFMs，其16S rRNA基因序列分别与两种不同的甲基杆菌属物种，即藤泽甲基杆菌（M. fujisawaense DSM5686(T)）和耐辐射甲基杆菌（M. radiotolerans JCM2831(T)）具有高度相似性，这表明紫苏与PPFMs之间存在特定的相互作用。
