National Bureau of Agriculturally Important Microorganisms, Kushmaur, Maunath Bhanjan, India.
Antonie Van Leeuwenhoek. 2012 May;101(4):777-86. doi: 10.1007/s10482-011-9692-9. Epub 2011 Dec 27.
Methylotrophic bacteria were isolated from the phyllosphere of different crop plants such as sugarcane, pigeonpea, mustard, potato and radish. The methylotrophic isolates were differentiated based on growth characteristics and colony morphology on methanol supplemented ammonium mineral salts medium. Amplification of the mxaF gene helped in the identification of the methylotrophic isolates as belonging to the genus Methylobacterium. Cell-free culture filtrates of these strains enhanced seed germination of wheat (Triticum aestivum) with highest values of 98.3% observed using Methylobacterium sp. (NC4). Highest values of seedling length and vigour were recorded with Methylobacterium sp. (NC28). HPLC analysis of production by bacterial strains ranged from 1.09 to 9.89 μg ml(-1) of cytokinins in the culture filtrate. Such cytokinin producing beneficial methylotrophs can be useful in developing bio-inoculants through co-inoculation of pink-pigmented facultative methylotrophs with other compatible bacterial strains, for improving plant growth and productivity, in an environment-friendly manner.
从甘蔗、木豆、芥菜、土豆和萝卜等不同作物的叶际中分离出了甲基营养细菌。根据在补充甲醇的铵盐矿物盐培养基上的生长特性和菌落形态，对甲基营养分离物进行了区分。扩增 mxaF 基因有助于鉴定甲基营养分离物属于甲基杆菌属。这些菌株的无细胞培养滤液促进了小麦（Triticum aestivum）的种子萌发，使用甲基杆菌（NC4）观察到最高值为 98.3%。用甲基杆菌（NC28）记录到最高的幼苗长度和活力值。通过 HPLC 分析，细菌菌株在培养滤液中的细胞分裂素产量范围为 1.09 至 9.89 μg ml(-1)。这种产生细胞分裂素的有益甲基营养菌可通过与其他相容的细菌菌株共同接种粉红着色兼性甲基营养菌，以环保的方式促进植物生长和生产力，从而用于开发生物接种剂。
