Elchaninov A V, Bolshakova G B
Laboratory of Growth and Development, Institute of Human Morphology, the Russian Academy of Medical Sciences, Moscow, Russia.
Bull Exp Biol Med. 2011 Jul;151(3):374-7. doi: 10.1007/s10517-011-1334-8.
We studied hepatocyte proliferation in the regenerating liver of 17-day fetuses of outbred albino rats. The animals were sacrificed every 3 h over 2 days after resection of 20% liver. The number of mitoses beyond the zone of injury increased sharply 12 and 24 h after resection. The hepatocyte mitotic index in the perifocal zone did not surpass the mitotic index in regions distant from the focus of injury during any of the studied periods. No circadian rhythm of hepatocyte mitotic activity was detected for resected or intact fetal liver. The injury caused virtually no changes in hepatocyte mitosis phase ratio in the operated compared to intact liver, which attested to stable course of mitosis. The weight of fetal liver recovered at the expense of enhanced mitotic activity of hepatocytes in the entire liver.
我们研究了远交系白化大鼠17日龄胎儿再生肝脏中的肝细胞增殖情况。在切除20%肝脏后，每隔3小时处死一批动物，持续2天。切除后12小时和24小时，损伤区域以外的有丝分裂数量急剧增加。在任何研究时间段内，病灶周围区域的肝细胞有丝分裂指数均未超过远离损伤病灶区域的有丝分裂指数。对于切除或完整的胎儿肝脏，均未检测到肝细胞有丝分裂活性的昼夜节律。与完整肝脏相比，手术造成的损伤实际上并未改变肝细胞有丝分裂期的比例，这证明有丝分裂过程稳定。胎儿肝脏的重量通过整个肝脏中肝细胞有丝分裂活性的增强得以恢复。
