Souto M, Llanos J M
Instituto de Embriología, Biología e Histología, Facultad de Ciencias Médicas, Universidad Nacional de La Plata, Argentina.
Chronobiol Int. 1985;2(3):169-75. doi: 10.3109/07420528509055556.
Standardized (light from 0600 to 1800) C3HS mice, hepatectomized at different circadian stages, were killed at 1400 (the peak time of mitotic activity in intact mice). The higher values of mitotic index were those of mice operated at 1400, 48 hr before. The curve of mitotic activity of the regenerating liver of mice operated at 1400 and that of mice operated at 0200 (an opposite time in the circadian stage) are, both, grossly in phase with the curves of mitotic index in young and adult mice liver. The amplitude of the first peak of mitotic activity in mice operated at 0200 was dramatically lower than that of animals operated at 1400. The same applies to hepatocytes as well as to the sinusoid litoral population of cells. It is concluded that 1400 hr, as contrast to 0200 hr, is an optimal time for hepatectomy if one wants to obtain the highest mitotic index first peak during regeneration in a normal phase position (the position of the mitotic index peak in the liver of normal young and adult mice).
标准化（0600至1800光照）的C3HS小鼠，在不同昼夜节律阶段进行肝切除，于1400（正常小鼠有丝分裂活动的峰值时间）处死。有丝分裂指数较高的值是那些在48小时前的1400进行手术的小鼠。在1400进行手术的小鼠再生肝的有丝分裂活动曲线以及在0200（昼夜节律阶段的相反时间）进行手术的小鼠的曲线，总体上都与幼龄和成年小鼠肝脏中有丝分裂指数的曲线同步。在0200进行手术的小鼠有丝分裂活动的第一个峰值幅度明显低于在1400进行手术的动物。这同样适用于肝细胞以及肝窦边缘细胞群体。得出的结论是，如果想要在正常相位位置（正常幼龄和成年小鼠肝脏中有丝分裂指数峰值的位置）的再生过程中首先获得最高的有丝分裂指数峰值，与0200时相比，1400时是肝切除的最佳时间。
