Guangdong Provincial Key Laboratory of Chemical Biology, Graduate School at Shenzhen, Tsinghua University, Shenzhen, 518055, P. R. China.
Analyst. 2012 May 21;137(10):2309-12. doi: 10.1039/c2an16092g. Epub 2012 Mar 27.
Label-free fluorescent assays were developed based on the competition of intramolecular DNA hybridization and aptamer-target binding. Using small molecule adenosine triphosphate (ATP) and biomacro-molecule thrombin as model targets, our design was proved to be a general method with good sensitivity and high selectivity.
基于分子内 DNA 杂交和适体与靶标结合的竞争，开发了无标记荧光检测法。以小分子三磷酸腺苷（ATP）和生物大分子凝血酶为模型靶标，证实了我们的设计是一种具有良好灵敏度和高选择性的通用方法。
