Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Materials Science, Shaanxi Normal University, Xi'an 710062, People's Republic of China.
Biosens Bioelectron. 2011 Apr 15;26(8):3505-10. doi: 10.1016/j.bios.2011.01.035. Epub 2011 Mar 2.
We have developed a new multicolor fluorescent sensing system to detect multiple analytes in one pot. This design is based on the noncovalent assembly of dye-labeled aptamer with single-walled carbon nanotubes (SWNTs) by π-stacking between the nucleotide bases and the SWNTs sidewalls. In the presence of the targets, the aptamer-target binding separates the assembly of dye-labeled aptamers and SWNTs, resulting in the restoration of fluorescence signal of the dye labeled with aptamers. As a proof of concept, we demonstrate that a two-color fluorescent system can simultaneously and selectively detect two targets (thrombin and adenosine triphosphate) in a single solution. Since the method is mix-and-detect manner, the present strategy is simple and cost-effective.
我们开发了一种新的多色荧光传感系统，可以在一个小瓶中同时检测多种分析物。该设计基于通过核苷酸碱基与单壁碳纳米管 (SWNTs) 侧壁之间的π-堆积，将染料标记的适体与单壁碳纳米管 (SWNTs) 非共价组装。在存在靶标时，适体-靶标结合将染料标记的适体与 SWNTs 的组装分离，导致染料标记的适体的荧光信号恢复。作为概念验证，我们证明了双色荧光系统可以在单一溶液中同时选择性地检测两种靶标（凝血酶和三磷酸腺苷）。由于该方法是混合检测方式，因此本策略简单且具有成本效益。
