Department of Cancer Prevention and Control, Roswell Park Cancer Institute, Buffalo, NY 14263, USA.
Crit Rev Food Sci Nutr. 2012;52(6):525-32. doi: 10.1080/10408398.2010.503288.
Many naturally occurring isothiocyanates (ITCs) show highly promising chemopreventive activities. Humans are commonly exposed to these compounds through the consumption of cruciferous vegetables which are the main source of dietary ITCs. Dietary ITCs may play an important role in cancer prevention and in the well-recognized cancer preventive activities of cruciferous vegetables. A generic analytical method, namely the 1,2-benzenedithiol-based cyclocondensation assay, was previously developed for quantitation of ITCs and their in vivo metabolites. This method has been widely used and has contributed greatly to research on chemoprevention by ITCs. In this article, the discovery and development of the cyclocondensation assay are recapitulated, and its sensitivity and specificity as well as its advantages and limitations are scrutinized. Moreover, detailed discussion is also provided to show how this assay has been used to advance our understanding of the cancer chemopreventive potential and the mechanism of action of ITCs.
许多天然存在的异硫氰酸酯（ITCs）表现出极具前景的化学预防活性。人类通常通过食用十字花科蔬菜来接触这些化合物，十字花科蔬菜是膳食 ITCs 的主要来源。膳食 ITCs 可能在癌症预防和十字花科蔬菜已被公认的癌症预防活动中发挥重要作用。一种通用的分析方法，即基于 1,2-苯二硫醇的环缩合测定法，以前曾用于 ITCs 及其体内代谢物的定量。该方法已被广泛应用，并为 ITCs 的化学预防研究做出了巨大贡献。本文回顾了环缩合测定法的发现和发展，考察了其灵敏度和特异性，以及其优缺点。此外，还详细讨论了如何使用该测定法来加深我们对 ITCs 的癌症化学预防潜力和作用机制的理解。
