Doria Enrico, Buonocore Daniela, Marra Antonio, Bontà Valeria, Gazzola Andrea, Dossena Maurizia, Verri Manuela, Calvio Cinzia
Department of Biology and Biotechnology L. Spallanzani, University of Pavia, 27100 Pavia, Italy.
Department of Earth and Environmental Sciences, University of Pavia, 27100 Pavia, Italy.
Plants (Basel). 2022 Mar 18;11(6):816. doi: 10.3390/plants11060816.
The market for nutraceutical molecules is growing at an impressive pace in all Western countries. A convenient source of bioactive compounds is found in vegetable waste products, and their re-use for the recovery of healthy biomolecules would increase the sustainability of the food production system. However, safe, cheap, and sustainable technologies should be applied for the recovery of these beneficial molecules, avoiding the use of toxic organic solvents or expensive equipment. The soil bacterium is naturally endowed with several enzymes targeting complex vegetable polymers. In this work, a raw bacterial culture supernatant was used to assist in the extraction of bioactives using isothermal pressurization cycles. Besides a wild-type strain, a new strain showing increased secretion of cellulases and xylanases, pivotal enzymes for the digestion of the plant cell wall, was also used. Results indicate that the recovery of compounds correlates with the amount of cellulolytic enzymes applied, demonstrating that the pretreatment with non-purified culture broth effectively promotes the release of bioactives from the vegetable matrix. Therefore, this approach is a valid and sustainable procedure for the recovery of bioactive compounds from food waste.
在所有西方国家，营养保健品分子市场正以惊人的速度增长。植物废弃物是生物活性化合物的便捷来源，将其重新用于健康生物分子的回收将提高食品生产系统的可持续性。然而，应采用安全、廉价且可持续的技术来回收这些有益分子，避免使用有毒有机溶剂或昂贵设备。土壤细菌天然具有多种针对复杂植物聚合物的酶。在这项工作中，使用原始细菌培养上清液通过等温加压循环辅助生物活性物质的提取。除了野生型菌株外，还使用了一种新菌株，该菌株显示出纤维素酶和木聚糖酶的分泌增加，这两种酶是消化植物细胞壁的关键酶。结果表明，化合物的回收率与所应用的纤维素分解酶的量相关，表明用未纯化的培养液进行预处理可有效促进生物活性物质从植物基质中释放出来。因此，这种方法是从食物废料中回收生物活性化合物的一种有效且可持续的方法。
