División de Neurociencias, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, Apartado Postal 70-253, 04510 México D.F., Mexico.
Neurobiol Learn Mem. 2012 May;97(4):418-24. doi: 10.1016/j.nlm.2012.03.006. Epub 2012 Mar 20.
In this work we probed the effects of post-trial infusions of the muscarinic receptor antagonist scopolamine on object recognition memory formation. Scopolamine was infused bilaterally immediately after the sample phase in the perirhinal cortex or dorsal hippocampus and animals were tested for short-term (90 min) or long-term (24 h) memory. Results showed that scopolamine impaired short-term memory when injected in either the perirhinal cortex or hippocampus. Nevertheless, scopolamine disrupted long-term memory when administrated in the perirhinal cortex but not when applied in the hippocampus. Long-term memory was unaffected when scopolamine was infused 160 min after the sample phase or 90 min before test phase. Our data indicate that short-term recognition memory requires muscarinic receptors signaling in both the perirhinal cortex and hippocampus, whereas long-term recognition memory depends on muscarinic receptors in the perirhinal cortex but not hippocampus. These results support a differential involvement of muscarinic activity in these two medial temporal lobe structures in the formation of recognition memory.
在这项工作中，我们探讨了在样本阶段后双侧注射毒蕈碱受体拮抗剂东莨菪碱对物体识别记忆形成的影响。东莨菪碱在双侧内嗅皮层或背侧海马体中立即输注，动物在短期（90 分钟）或长期（24 小时）记忆测试中进行测试。结果表明，当在内嗅皮层或海马体中注射东莨菪碱时，会损害短期记忆。然而，当在内嗅皮层中给予东莨菪碱时，会破坏长期记忆，但当在海马体中应用时不会破坏。当在样本阶段后 160 分钟或测试阶段前 90 分钟输注东莨菪碱时，长期记忆不受影响。我们的数据表明，短期识别记忆需要内嗅皮层和海马体中的毒蕈碱受体信号，而长期识别记忆则依赖于内嗅皮层中的毒蕈碱受体，但不依赖于海马体中的毒蕈碱受体。这些结果支持在这两个内侧颞叶结构中，毒蕈碱活性在识别记忆形成中具有不同的参与。
